Development of a Preclinical Therapeutic Model of Human Brain Metastasis with Chemoradiotherapy
Martínez Aranda, Antonio (Institut d'Investigació Biomèdica de Bellvitge)
Hernández, Vanessa (Institut d'Investigació Biomèdica de Bellvitge)
Picón, Cristina (Institut Català d'Oncologia)
Modolell, Ignasi (Institut Català d'Oncologia)
Sierra, Angels (Institut d'Investigació Biomèdica de Bellvitge)

Fecha:	2013
Resumen:	Currently, survival of breast cancer patients with brain metastasis ranges from 2 to 16 months. In experimental brain metastasis studies, only 10% of lesions with the highest permeability exhibited cytotoxic responses to paclitaxel or doxorubicin. Therefore, radiation is the most frequently used treatment, and sensitizing agents, which synergize with radiation, can improve the efficacy of the therapy. In this study we used 435-Br1 cells containing the fluorescent protein (eGFP) gene and the photinus luciferase (PLuc) gene to develop a new brain metastatic cell model in mice through five in vivo/in vitro rounds. BR-eGFP-CMV/Luc-V5 brain metastatic cells induce parenchymal brain metastasis within 60. 8 ± 13. 8 days of intracarotid injection in all mice. We used this model to standardize a preclinical chemoradiotherapy protocol comprising three 5. 5 Gy fractions delivered on consecutive days (overall dose of 16. 5 Gy) which improved survival with regard to controls (60. 29 ± 8. 65 vs. 47. 20 ± 11. 14). Moreover, the combination of radiotherapy with temozolomide, 60 mg/Kg/day orally for five consecutive days doubled survival time of the mice 121. 56 ± 52. 53 days (Kaplan-Meier Curve, p < 0. 001). This new preclinical chemoradiotherapy protocol proved useful for the study of radiation response/resistance in brain metastasis, either alone or in combination with new sensitizing agents. Currently, survival of breast cancer patients with brain metastasis ranges from 2 to 16 months. In experimental brain metastasis studies, only 10% of lesions with the highest permeability exhibited cytotoxic responses to paclitaxel or doxorubicin. Therefore, radiation is the most frequently used treatment, and sensitizing agents, which synergize with radiation, can improve the efficacy of the therapy. In this study we used 435-Br1 cells containing the fluorescent protein (eGFP) gene and the photinus luciferase (PLuc) gene to develop a new brain metastatic cell model in mice through five in vivo/in vitro rounds. BR-eGFP-CMV/Luc-V5 brain metastatic cells induce parenchymal brain metastasis within 60. 8 ± 13. 8 days of intracarotid injection in all mice. We used this model to standardize a preclinical chemoradiotherapy protocol comprising three 5. 5 Gy fractions delivered on consecutive days (overall dose of 16. 5 Gy) which improved survival with regard to controls (60. 29 ± 8. 65 vs. 47. 20 ± 11. 14). Moreover, the combination of radiotherapy with temozolomide, 60 mg/Kg/day orally for five consecutive days doubled survival time of the mice 121. 56 ± 52. 53 days (Kaplan-Meier Curve, p < 0. 001). This new preclinical chemoradiotherapy protocol proved useful for the study of radiation response/resistance in brain metastasis, either alone or in combination with new sensitizing agents.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Brain metastasis ; Breast cancer ; Experimental models ; Radiation ; Temozolomide ; Therapy
Publicado en:	International journal of molecular sciences, Vol. 14 (2013) , p. 8306-8327, ISSN 1422-0067

DOI: 10.3390/ijms14048306
PMID: 23591844

22 p, 2.0 MB

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