Development of a transplantable glioma tumour model from genetically engineered mice : MRI/MRS/MRSI characterisation
Ciezka, Magdalena (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Acosta González, Milena (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Herranz, Cristina (Universitat de Barcelona. Departament de Ciències Biomèdiques)
Canals, Josep M. (Universitat de Barcelona. Departament de Ciències Biomèdiques)
Pumarola i Batlle, Martí (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Candiota Silveira, Ana Paula (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Arús i Caraltó, Carles (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Fecha:	2016
Resumen:	The initial aim of this study was to generate a transplantable glial tumour model of low-intermediate grade by disaggregation of a spontaneous tumour mass from genetically engineered models (GEM). This should result in an increased tumour incidence in comparison to GEM animals. An anaplastic oligoastrocytoma (OA) tumour of World Health Organization (WHO) grade III was obtained from a female GEM mouse with the S100β-v-erbB/inK4a-Arf (+/-) genotype maintained in the C57BL/6 background. The tumour tissue was disaggregated; tumour cells from it were grown in aggregates and stereotactically injected into C57BL/6 mice. Tumour development was followed using Magnetic Resonance Imaging (MRI), while changes in the metabolomics pattern of the masses were evaluated by Magnetic Resonance Spectroscopy/Spectroscopic Imaging (MRS/MRSI). Final tumour grade was evaluated by histopathological analysis. The total number of tumours generated from GEM cells from disaggregated tumour (CDT) was 67 with up to 100 % penetrance, as compared to 16 % in the local GEM model, with an average survival time of 66 ± 55 days, up to 4. 3-fold significantly higher than the standard GL261 glioblastoma (GBM) tumour model. Tumours produced by transplantation of cells freshly obtained from disaggregated GEM tumour were diagnosed as WHO grade III anaplastic oligodendroglioma (ODG) and OA, while tumours produced from a previously frozen sample were diagnosed as WHO grade IV GBM. We successfully grew CDT and generated tumours from a grade III GEM glial tumour. Freezing and cell culture protocols produced progression to grade IV GBM, which makes the developed transplantable model qualify as potential secondary GBM model in mice.
Ayudas:	Ministerio de Ciencia e Innovación SAF2011-23870 Ministerio de Economía y Competitividad SAF2012-37417 Ministerio de Economía y Competitividad SAF2014-52332-R
Derechos:	Aquest material està protegit per drets d'autor i/o drets afins. Podeu utilitzar aquest material en funció del que permet la legislació de drets d'autor i drets afins d'aplicació al vostre cas. Per a d'altres usos heu d'obtenir permís del(s) titular(s) de drets.
Documento:	Article
Materia:	Preclinical brain tumour ; Molecular imaging ; Secondary glioblastoma ; Disaggregated tumour
Publicado en:	Journal of neuro-oncology, Vol. 129, issue 1 (Aug. 2016), p. 67-76, ISSN 0167-594X

Post-print 59 p, 12.4 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Unitat de Patologia Murina i Comparada
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