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Gene expression profiling and association with prion-related lesions in the medulla oblongata of symptomatic natural scrapie animals
Filalil, Hicham (Universidad de Zaragoza. Facultad de Veterinaria)
Martín Burriel, Inmaculada (Universidad de Zaragoza. Facultad de Veterinaria)
Harders, Frank (Central Institute for Animal Disease Control (Lelystad, Països Baixos))
Varona, Luis (Universidad de Zaragoza. Facultad de Veterinaria)
Lyahyai, Jaber (Universidad de Zaragoza. Facultad de Veterinaria)
Zaragoza Fernández, Pilar (Universidad de Zaragoza. Facultad de Veterinaria)
Pumarola i Batlle, Martí (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Badiola, Juan J. (Universidad de Zaragoza. Facultad de Veterinaria)
Bossers, Alex (Central Institute for Animal Disease Control (Lelystad, Països Baixos))
Bolea, Rosa (Universidad de Zaragoza. Facultad de Veterinaria)

Fecha: 2011
Resumen: The pathogenesis of natural scrapie and other prion diseases remains unclear. Examining transcriptome variations in infected versus control animals may highlight new genes potentially involved in some of the molecular mechanisms of prion-induced pathology. The aim of this work was to identify disease-associated alterations in the gene expression profiles of the caudal medulla oblongata (MO) in sheep presenting the symptomatic phase of natural scrapie. The gene expression patterns in the MO from 7 sheep that had been naturally infected with scrapie were compared with 6 controls using a Central Veterinary Institute (CVI) custom designed 4644K microarray. The microarray consisted of a probe set on the previously sequenced ovine tissue library by CVI and was supplemented with all of the Ovis aries transcripts that are currently publicly available. Over 350 probe sets displayed greater than 2-fold changes in expression. We identified 148 genes from these probes, many of which encode proteins that are involved in the immune response, ion transport, cell adhesion, and transcription. Our results confirm previously published gene expression changes that were observed in murine models with induced scrapie. Moreover, we have identified new genes that exhibit differential expression in scrapie and could be involved in prion neuropathology. Finally, we have investigated the relationship between gene expression profiles and the appearance of the main scrapie-related lesions, including prion protein deposition, gliosis and spongiosis. In this context, the potential impacts of these gene expression changes in the MO on scrapie development are discussed.
Derechos: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Lengua: Anglès
Documento: article ; recerca ; publishedVersion
Materia: Malalties priòniques en els animals ; Prion diseases ; Scrapie
Publicado en: PloS one, Vol. 6 Issue 5 (May 2011) , p. 1-12, ISSN 1932-6203

DOI: 10.1371/journal.pone.0019909
PMID: 21629698

12 p, 953.8 KB

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