14-3-3 Proteins Participate in Light Signaling through Association with PHYTOCHROME INTERACTING FACTORs
Adams, Eri (RIKEN Center for Sustainable Resource Science (Japó))
Diaz, Celine (Centre de Recerca en Agrigenòmica)
Hong, Jong-Pil (RIKEN Center for Sustainable Resource Science (Japó))
Shin, Ryoung (RIKEN Center for Sustainable Resource Science (Japó))
Date: |
2014 |
Abstract: |
14-3-3 proteins are regulatory proteins found in all eukaryotes and are known to selectively interact with phosphorylated proteins to regulate physiological processes. Through an affinity purification screening, many light-related proteins were recovered as 14-3-3 candidate binding partners. Yeast two-hybrid analysis revealed that the 14-3-3 kappa isoform (14-3-3κ) could bind to PHYTOCHROME INTERACTING FACTOR3 (PIF3) and CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1). Further analysis by in vitro pull-down assay confirmed the interaction between 14-3-3κ and PIF3. Interruption of putative phosphorylation sites on the 14-3-3 binding motifs of PIF3 was not suficient to inhibit 14-3-3κ from binding or to disturb nuclear localization of PIF3. It was also indicated that 14-3-3κ could bind to other members of the PIF family, such as PIF1 and PIF6, but not to LONG HYPOCOTYL IN FAR-RED1 (HFR1). 14-3-3 mutants, as well as the PIF3 overexpressor, displayed longer hypocotyls, and a pif3 mutant displayed Shorter hypocotyls than the wild-type in red light, suggesting that 14-3-3 proteins are positive regulators of photomorphogenesis and function antagonistically with PIF3. Consequently, our results indicate that 14-3-3 proteins bind to PIFs and initiate photomorphogenesis in response to a light signal. |
Rights: |
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Language: |
Anglès |
Document: |
Article ; recerca ; Versió publicada |
Subject: |
PHYTOCHROME INTERACTING FACTOR (PIF) ;
14-3-3 protein ;
Light signaling ;
Photomorphogenesis ;
Arabidopsis thaliana ;
Plant ;
Protein-protein interaction |
Published in: |
International journal of molecular sciences, Vol. 15 Núm. 12 (december 2014) , p. 22801-22814, ISSN 1422-0067 |
DOI: 10.3390/ijms151222801
PMID: 25501334
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Record created 2016-12-12, last modified 2024-06-01