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Live attenuated African swine fever viruses as ideal tools to dissect the mechanisms involved in viral pathogenesis and immune protection
Lacasta, Anna (Fundació Centre de Recerca en Sanitat Animal)
Monteagudo, Paula L. (Universitat Autònoma de Barcelona. Fundació Centre de Recerca en Sanitat Animal)
Jiménez‑Marín, Ángeles
Accensi Alemany, Francesc (Fundació Centre de Recerca en Sanitat Animal)
Ballester Devis, Maria (Fundació Centre de Recerca en Sanitat Animal)
Argilaguet, Jordi M.
Galindo Cardiel, Iván (Fundació Centre de Recerca en Sanitat Animal)
Segalés Coma, Joaquim (Fundació Centre de Recerca en Sanitat Animal)
Salas, María L.
Domínguez Juncal, Javier
Moreno, Ángela
Garrido, Juan J.
Rodríguez González, Fernando (Fundació Centre de Recerca en Sanitat Animal)

Fecha: 2015
Resumen: African swine fever virus (ASFV) is the causal agent of African swine fever, a hemorrhagic and often lethal porcine disease causing enormous economical losses in affected countries. Endemic for decades in most of the sub-Saharan countries and Sardinia, the risk of ASFV-endemicity in Europe has increased since its last introduction into Europe in 2007. Live attenuated viruses have been demonstrated to induce very efficient protective immune responses, albeit most of the time protection was circumscribed to homologous ASFV challenges. However, their use in the field is still far from a reality, mainly due to safety concerns. In this study we compared the course of the in vivo infection caused by two homologous ASFV strains: the virulent E75 and the cell cultured adapted strain E75CV1, obtained from adapting E75 to grow in the CV1 cell-line. Interestingly, the kinetics of both viruses not only differed on the clinical signs that they caused and in the virus loads found, but also in the immunological pathways activated throughout the infections. Furthermore, E75CV1 confirmed its protective potential against the homologous E75 virus challenge and allowed the demonstration of poor cross-protection against BA71, thus defining it as heterologous. The in vitro specificity of the CD8+ T-cells present at the time of lethal challenge showed a clear activation against the homologous virus (E75) but not against BA71. These findings will be of utility for a better understanding of ASFV pathogenesis and for the rational designing of safe and efficient vaccines against this virus.
Ayudas: Ministerio de Economía y Competitividad AGL2010/22229
Ministerio de Economía y Competitividad AGL2013/48998C21R
Derechos: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Lengua: Anglès
Documento: Article ; recerca ; Versió publicada
Publicado en: Veterinary research, Vol. 46 (November 2015) , p. 1-16, ISSN 1297-9716

DOI: 10.1186/s13567-015-0275-z
PMID: 26589145

16 p, 2.0 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
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