Web of Science: 7 citas, Scopus: 6 citas, Google Scholar: citas,
Classical Swine Fever Virus vs. Classical Swine Fever Virus : the Superinfection Exclusion Phenomenon in Experimentally Infected Wild Boar
Muñoz González, Sara (Universitat Autònoma de Barcelona. Fundació Centre de Recerca en Sanitat Animal)
Pérez Simó, Marta (Universitat Autònoma de Barcelona. Fundació Centre de Recerca en Sanitat Animal)
Colom-Cadena, Andreu (Universitat Autònoma de Barcelona. Fundació Centre de Recerca en Sanitat Animal)
Cabezón Ponsoda, Óscar (Universitat Autònoma de Barcelona. Fundació Centre de Recerca en Sanitat Animal)
Bohórquez, José Alejandro (Universitat Autònoma de Barcelona. Fundació Centre de Recerca en Sanitat Animal)
Rosell, Rosa (Universitat Autònoma de Barcelona. Fundació Centre de Recerca en Sanitat Animal)
Pérez, Lester Josué
Marco Sánchez, Ignasi (Universitat Autònoma de Barcelona. Servei d'Ecopatologia de Fauna Salvatge)
Lavín González, Santiago (Universitat Autònoma de Barcelona. Servei d'Ecopatologia de Fauna Salvatge)
Domingo Álvarez, Mariano (Universitat Autònoma de Barcelona. Fundació Centre de Recerca en Sanitat Animal)
Ganges Espinosa, Llilianne (Universitat Autònoma de Barcelona. Fundació Centre de Recerca en Sanitat Animal)

Fecha: 2016
Resumen: Two groups with three wild boars each were used: Group A (animals 1 to 3) served as the control, and Group B (animals 4 to 6) was postnatally persistently infected with the Cat01 strain of CSFV (primary virus). The animals, six weeks old and clinically healthy, were inoculated with the virulent strain Margarita (secondary virus). For exclusive detection of the Margarita strain, a specific qRT-PCR assay was designed, which proved not to have cross-reactivity with the Cat01 strain. The wild boars persistently infected with CSFV were protected from superinfection by the virulent CSFV Margarita strain, as evidenced by the absence of clinical signs and the absence of Margarita RNA detection in serum, swabs and tissue samples. Additionally, in PBMCs, a well-known target for CSFV viral replication, only the primary infecting virus RNA (Cat01 strain) could be detected, even after the isolation in ST cells, demonstrating SIE at the tissue level in vivo. Furthermore, the data analysis of the Margarita qRT-PCR, by means of calculated ΔCt values, supported that PBMCs from persistently infected animals were substantially protected from superinfection after in vitro inoculation with the Margarita virus strain, while this virus was able to infect naive PBMCs efficiently. In parallel, IFN-α values were undetectable in the sera from animals in Group B after inoculation with the CSFV Margarita strain. Furthermore, these animals were unable to elicit adaptive humoral (no E2-specific or neutralising antibodies) or cellular immune responses (in terms of IFN-γ-producing cells) after inoculation with the second virus. Finally, a sequence analysis could not detect CSFV Margarita RNA in the samples tested from Group B. Our results suggested that the SIE phenomenon might be involved in the evolution and phylogeny of the virus, as well as in CSFV control by vaccination. To the best of our knowledge, this study was one of the first showing efficient suppression of superinfection in animals, especially in the absence of IFN-α, which might be associated with the lack of innate immune mechanisms.
Derechos: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Lengua: Anglès.
Documento: article ; recerca ; publishedVersion
Materia: RNA extraction ; Swine ; Superinfection ; Enzyme-linked immunoassays ; Sequence analysis ; Viral persistence and latency ; Immune response ; RNA viruses
Publicado en: PLoS one, Vol. 11 Núm. 2 (February 2016) , p. 1-19, ISSN 1932-6203

DOI: 10.1371/journal.pone.0149469
PMID: 26919741


19 p, 751.7 KB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Artículos > Artículos de investigación
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 Registro creado el 2017-02-27, última modificación el 2018-10-27



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