European Sea Bass (Dicentrarchus labrax) Immune Status and Disease Resistance Are Impaired by Arginine Dietary Supplementation
Azeredo, Rita (Universidade do Porto. Centro Interdisciplinar de Investigação Marinha e Ambiental (CIMAR))
Pérez-Sánchez, Jaume (Instituto de Acuicultura de Torre de la Sal (Venta de San Antonio-Estación, Castelló))
Sitjà-Bobadilla, Ariadna (Instituto de Acuicultura de Torre de la Sal (Venta de San Antonio-Estación, Castelló))
Fouz, Belén (Universitat de València. Departament de Microbiologia i Ecologia)
Tort Bardolet, Lluís (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Aragão, Cláudia (Universidade do Algarve. Centro de Ciências do Mar)
Oliva-Teles, Aires (Universidade do Porto. Centro Interdisciplinar de Investigação Marinha e Ambiental (CIMAR))
Costas, Benjamín (Universidade do Porto. Centro Interdisciplinar de Investigação Marinha e Ambiental (CIMAR))

Date:	2015
Abstract:	Infectious diseases and fish feeds management are probably the major expenses in the aquaculture business. Hence, it is a priority to define sustainable strategies which simultaneously avoid therapeutic procedures and reinforce fish immunity. Currently, one preferred approach is the use of immunostimulants which can be supplemented to the fish diets. Arginine is a versatile amino acid with important mechanisms closely related to the immune response. Aiming at finding out how arginine affects the innate immune status or improve disease resistance of European seabass (Dicentrarchus labrax) against vibriosis, fish were fed two arginine-supplemented diets (1% and 2% arginine supplementation). A third diet meeting arginine requirement level for seabass served as control diet. Following 15 or 29 days of feeding, fish were sampled for blood, spleen and gut to assess cell-mediated immune parameters and immune-related gene expression. At the same time, fish from each dietary group were challenged against Vibrio anguillarum and survival was monitored. Cell-mediated immune parameters such as the extracellular superoxide and nitric oxide decreased in fish fed arginine-supplemented diets. Interleukins and immune-cell marker transcripts were down-regulated by the highest supplementation level. Disease resistance data were in accordance with a generally depressed immune status, with increased susceptibility to vibriosis in fish fed arginine supplemented diets. Altogether, these results suggest a general inhibitory effect of arginine on the immune defences and disease resistance of European seabass. Still, further research will certainly clarify arginine immunomodulation pathways thereby allowing the validation of its potential as a prophylactic strategy.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Arginine ; Diet ; Gene expression ; Spleen ; Respiratory burst ; Immune response ; Interleukins ; Polymerase chain reaction
Published in:	PloS one, Vol. 10 Num. 10 (October 2015) , p. 1-19, ISSN 1932-6203

DOI: 10.1371/journal.pone.0139967
PMID: 26447480

19 p, 920.1 KB

The record appears in these collections:
Articles > Research articles
Articles > Published articles