SOS system induction inhibits the assembly of chemoreceptor signaling clusters in Salmonella enterica
Irazoki, Oihane (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Mayola Coromina, Albert (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Campoy Sánchez, Susana (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)
Barbé García, Jordi (Universitat Autònoma de Barcelona. Departament de Genètica i de Microbiologia)

Date:	2016
Abstract:	Swarming, a flagellar-driven multicellular form of motility, is associated with bacterial virulence and increased antibiotic resistance. In this work we demonstrate that activation of the SOS response reversibly inhibits swarming motility by preventing the assembly of chemoreceptor-signaling polar arrays. We also show that an increase in the concentration of the RecA protein, generated by SOS system activation, rather than another function of this genetic network impairs chemoreceptor polar cluster formation. Our data provide evidence that the molecular balance between RecA and CheW proteins is crucial to allow polar cluster formation in Salmonella enterica cells. Thus, activation of the SOS response by the presence of a DNA-injuring compound increases the RecA concentration, thereby disturbing the equilibrium between RecA and CheW and resulting in the cessation of swarming. Nevertheless, when the DNA-damage decreases and the SOS response is no longer activated, basal RecA levels and thus polar cluster assembly are reestablished. These results clearly show that bacterial populations moving over surfaces make use of specific mechanisms to avoid contact with DNA-damaging compounds.
Grants:	Ministerio de Economía y Competitividad BFU2011-23478 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-572
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	PloS one, Vol. 11 Núm. 1 (Gener 2016) , p. e0146685, ISSN 1932-6203

DOI: 10.1371/journal.pone.0146685
PMID: 26784887

22 p, 3.2 MB

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