Web of Science: 12 citations, Scopus: 11 citations, Google Scholar: citations,
Differential subplastidial localization and turnover of enzymes iInvolved in isoprenoid biosynthesis in chloroplasts
Perello, Catalina (Centre de Recerca en Agrigenòmica)
Llamas, Ernesto (Centre de Recerca en Agrigenòmica)
Burlat, Vincent (Centre national de la recherche scientifique (França))
Ortiz-Alcaide, Miriam (Centre de Recerca en Agrigenòmica)
Phillips, Michael A. (Centre de Recerca en Agrigenòmica)
Pulido, Pablo (Centre de Recerca en Agrigenòmica)
Rodríguez Concepción, Manuel (Centre de Recerca en Agrigenòmica)

Date: 2016
Abstract: Plastidial isoprenoids are a diverse group of metabolites with roles in photosynthesis, growth regulation, and interaction with the environment. The methylerythritol 4-phosphate (MEP) pathway produces the metabolic precursors of all types of plastidial isoprenoids. Proteomics studies in Arabidopsis thaliana have shown that all the enzymes of the MEP pathway are localized in the plastid stroma. However, immunoblot analysis of chloroplast subfractions showed that the first two enzymes of the pathway, deoxyxylulose 5-phosphate synthase (DXS) and reductoisomerase (DXR), can also be found in non-stromal fractions. Both transient and stable expression of GFP-tagged DXS and DXR proteins confirmed the presence of the fusion proteins in distinct subplastidial compartments. In particular, DXR-GFP was found to accumulate in relatively large vesicles that could eventually be released from chloroplasts, presumably to be degraded by an autophagy-independent process. Together, we propose that protein-specific mechanisms control the localization and turnover of the first two enzymes of the MEP pathway in Arabidopsis chloroplasts.
Note: Número d'acord de subvenció EC/FP7/245143
Note: Número d'acord de subvenció MINECO/BIO2015-71703-REDT
Note: Número d'acord de subvenció MINECO/BIO2014-59092-P
Note: Número d'acord de subvenció AGAUR/2014/SGR-1434
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Published in: Plos one, Vol. 11 Núm. 2 (2016) , e0150539, ISSN 1932-6203

DOI: 10.1371/journal.pone.0150539
PMID: 26919668


17 p, 3.6 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2017-06-14, last modified 2019-07-22



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