Production of BP178, a derivative of the synthetic antibacterial peptide BP100, in the rice seed endosperm
Montesinos Barreda, Laura (Universitat de Girona. Institut de Tecnologia Agroalimentària)
Bundó Barberà, Mireia (Centre de Recerca en Agrigenòmica)
Badosa Romañó, Esther (Universitat de Girona. Institut de Tecnologia Agroalimentària)
San Segundo, Blanca (Centre de Recerca en Agrigenòmica)
Coca López, María (Centre de Recerca en Agrigenòmica)
Montesinos Seguí, Emilio (Universitat de Girona. Institut de Tecnologia Agroalimentària)

Date:	2017
Abstract:	Background: BP178 peptide is a synthetic BP100-magainin derivative possessing strong inhibitory activity against plant pathogenic bacteria, offering a great potential for future applications in plant protection and other fields. Here we report the production and recovery of a bioactive BP178 peptide using rice seeds as biofactories. - Results: a synthetic gene encoding the BP178 peptide was prepared and introduced in rice plants. The gene was efficiently expressed in transgenic rice under the control of an endosperm-specific promoter. Among the three endosperm-specific rice promoters (Glutelin B1, Glutelin B4 or Globulin 1), best results were obtained when using the Globulin 1 promoter. The BP178 peptide accumulated in the seed endosperm and was easily recovered from rice seeds using a simple procedure with a yield of 21 μg/g. The transgene was stably inherited for at least three generations, and peptide accumulation remained stable during long term storage of transgenic seeds. The purified peptide showed in vitro activity against the bacterial plant pathogen Dickeya sp. , the causal agent of the dark brown sheath rot of rice. Seedlings of transgenic events showed enhanced resistance to the fungal pathogen Fusarium verticillioides, supporting that the in planta produced peptide was biologically active. -Conclusions: the strategy developed in this work for the sustainable production of BP178 peptide using rice seeds as biofactories represents a promising system for future production of peptides for plant protection and possibly in other fields.
Grants:	Ministerio de Ciencia e Innovación EUI2008-03769 Ministerio de Ciencia e Innovación EUI2008-03572 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014-SGR-697 Agència de Gestió d'Ajuts Universitaris i de Recerca 2008-SGR-812 Agència de Gestió d'Ajuts Universitaris i de Recerca SGR-09626
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Antimicrobial peptide ; Oryza sativa ; Rice biofactory ; Protein bodies ; Peptide recovery ; Pathogen resistance
Published in:	BMC plant biology, Vol. 17 (March 2017) , art 63, ISSN 1471-2229

DOI: 10.1186/s12870-017-1011-9
PMID: 28292258

14 p, 3.7 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles