The HY5-PIF regulatory module coordinates light and temperature control of photosynthetic gene transcription
Toledo-Ortiz, Gabriela (University of Edinburgh. Institute of Structural and Molecular Biology)
Johansson, Henrik (University of Edinburgh. Institute of Structural and Molecular Biology)
Lee, Keun Pyo (University of Edinburgh. Institute of Structural and Molecular Biology)
Bou Torrent, Jordi (Centre de Recerca en Agrigenòmica)
Stewart, Kelly (University of Edinburgh. Institute of Structural and Molecular Biology)
Steel, Gavin (University of Edinburgh. Institute of Structural and Molecular Biology)
Rodríguez Concepción, Manuel (Centre de Recerca en Agrigenòmica)
Halliday, Karen J. (University of Edinburgh. Institute of Structural and Molecular Biology)

Date:	2014
Abstract:	The ability to interpret daily and seasonal alterations in light and temperature signals is essential for plant survival. This is particularly important during seedling establishment when the phytochrome photoreceptors activate photosynthetic pigment production for photoautotrophic growth. Phytochromes accomplish this partly through the suppression of PHYTOCHROME INTERACTING FACTORS (PIFs), negative regulators of chlorophyll and carotenoid biosynthesis. While the bZIP transcription factor LONG HYPOCOTYL 5 (HY5), a potent PIF antagonist, promotes photosynthetic pigment accumulation in response to light. Here we demonstrate that by directly targeting a common promoter cis-element (G-box), HY5 and PIFs form a dynamic activation-suppression transcriptional module responsive to light and temperature cues. This antagonistic regulatory module provides a simple, direct mechanism through which environmental change can redirect transcriptional control of genes required for photosynthesis and photoprotection. In the regulation of photopigment biosynthesis genes, HY5 and PIFs do not operate alone, but with the circadian clock. However, sudden changes in light or temperature conditions can trigger changes in HY5 and PIFs abundance that adjust the expression of common target genes to optimise photosynthetic performance and growth.
Grants:	European Commission 245143 European Commission 321649 Ministerio de Economía y Competitividad BIO2011/23680 Ministerio de Economía y Competitividad PIM2010/00660 Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-26
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	PLOS genetics, Vol. 10, issue 6 (June 2014) , e1004416, ISSN 1553-7404

DOI: 10.1371/journal.pgen.1004416
PMID: 24922306

14 p, 1.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles