Web of Science: 12 citations, Scopus: 13 citations, Google Scholar: citations,
Rewiring carotenoid biosynthesis in plants using a viral vector
Majer, Eszter (Consejo Superior de Investigaciones Científicas (Espanya). Instituto de Biología Molecular y Celular de Plantas)
Llorente, Briardo (Centre de Recerca en Agrigenòmica)
Rodríguez Concepción, Manuel (Centre de Recerca en Agrigenòmica)
Daròs, José-Antonio (Consejo Superior de Investigaciones Científicas (Espanya). Instituto de Biología Molecular y Celular de Plantas)

Date: 2017
Abstract: Plants can be engineered to sustainably produce compounds of nutritional, industrial or pharmaceutical relevance. This is, however, a challenging task as extensive regulation of biosynthetic pathways often hampers major metabolic changes. Here we describe the use of a viral vector derived from Tobacco etch virus to express a whole heterologous metabolic pathway that produces the health-promoting carotenoid lycopene in tobacco tissues. The pathway consisted in three enzymes from the soil bacteria Pantoea ananatis. Lycopene is present at undetectable levels in chloroplasts of non-infected leaves. In tissues infected with the viral vector, however, lycopene comprised approximately 10% of the total carotenoid content. Our research further showed that plant viruses that express P. ananatis phytoene synthase (crtB), one of the three enzymes of the heterologous pathway, trigger an accumulation of endogenous carotenoids, which together with a reduction in chlorophylls eventually result in a bright yellow pigmentation of infected tissues in various host-virus combinations. So, besides illustrating the potential of viral vectors for engineering complex metabolic pathways, we also show a yellow carotenoid-based reporter that can be used to visually track infection dynamics of plant viruses either alone or in combination with other visual markers.
Note: Número d'acord de subvenció MINECO/BIO2015-71703-REDT
Note: Número d'acord de subvenció MINECO/BIO2014-59092-P
Note: Número d'acord de subvenció MINECO/BIO2014-54269-R
Note: Número d'acord de subvenció AGAUR/2014/SGR-1434
Note: Número d'acord de subvenció MINECO/FPDI-2013-018882
Note: Número d'acord de subvenció MECD/AP2012-3751
Note: Altres ajuts: De la Generalitat Valenciana (PROMETEOII/2014/021) i del Programa Iberoamericano de Ciencia y Tecnologia para el Desarrollo (Ibercarot 112RT0445).
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Molecular engineering in plants ; Viral vectors
Published in: Scientific reports, Vol. 7 (Jan. 2017) , art. 41645, ISSN 2045-2322

DOI: 10.1038/srep41645
PMID: 28139696

10 p, 1.3 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2017-07-24, last modified 2019-07-22

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