The over-expression of the plastidial transglutaminase from maize in Arabidopsis increases the activation threshold of photoprotection
Ioannidis, Nikolaos E. (University of Crete. Department of Biology)
Malliarakis, Dimitris (University of Crete. Department of Biology)
Torné, Josep M. (Centre de Recerca en Agrigenòmica)
Santos, Mireya (Centre de Recerca en Agrigenòmica)
Kotzabasis, Kiriakos (University of Crete. Department of Biology)

Date:	2016
Abstract:	Plastidial transglutaminase is one of the most promising enzymes in chloroplast bioenergetics due to its link with polyamine pathways and the cross talk with signals such as Ca2+ and GTP. Here, we show the effect of the increase of transglutaminase activity in Arabidopsis by using genetic transformation techniques. These lines fulfill their biological cycle normally (normal growth in soil, production of viable seeds) and show a relatively mild increase in transglutaminase activity (127%). These overexpressors of transglutaminase (OE TGase) have an extended stroma thylakoid network (71% higher number of PSIIβ centers), similar chlorophyll content (-4%), higher linear electron flow (+13%), and higher threshold of photoprotection activation (∼100%). On the other hand OE TGase showed a reduced maximum photochemistry of PSII (-6. 5%), a smaller antenna per photosystem II (-25%), a lower photoprotective "energization" quenching or qE (-77% at 490 μmol photons m-2 s-1) due to a higher threshold of qE activation and slightly lower light induced proton motive force (-17%). The role of the polyamines and of the transglutaminase in the regulation of chemiosmosis and photoprotection in chloroplasts is discussed.
Grants:	Ministerio de Ciencia e Innovación BFU2009-08575 Ministerio de Educación y Ciencia CSD2007/00036
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Polyamines ; Tolerance ; Photosynthesis ; Plant fitness ; Antenna regulation
Published in:	Frontiers in plant science, Vol. 7 (May 2016) , art. 635, ISSN 1664-462X

DOI: 10.3389/fpls.2016.00635
PMID: 27242838

13 p, 1.1 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles