Complex interplays between phytosterols and plastid development
Andrade, Paola (Centre de Recerca en Agrigenòmica)
Caudepón, Daniel (Centre de Recerca en Agrigenòmica)
Altabella Artigas, Teresa (Centre de Recerca en Agrigenòmica)
Arró i Plans, Montserrat (Centre de Recerca en Agrigenòmica)
Ferrer, Albert (Centre de Recerca en Agrigenòmica)
Manzano, David (Centre de Recerca en Agrigenòmica)

Fecha:	2017
Resumen:	Isoprenoids comprise the largest class of natural compounds and are found in all kinds of organisms. In plants, they participate in both primary and specialized metabolism, playing essential roles in nearly all aspects of growth and development. The enormous diversity of this family of compounds is extensively exploited for biotechnological and biomedical applications as biomaterials, biofuels or drugs. Despite their variety of structures, all isoprenoids derive from the common C₅ precursor isopentenyl diphosphate (IPP). Plants synthesize IPP through two different metabolic pathways, the mevalonic acid (MVA) and the 2-C-methyl-D-erythritol 4-phosphate (MEP) pathways that operate in the cytosol-RE and plastids, respectively. MEP-derived isoprenoids include important compounds for chloroplast function and as such, knock-out mutant plants affected in different steps of this pathway display important alterations in plastid structure. These alterations often lead to albino phenotypes and lethality at seedling stage. MVA knock-out mutant plants show, on the contrary, lethal phenotypes already exhibited at the gametophyte or embryo developmental stage. However, the recent characterization of conditional knock-down mutant plants of farnesyl diphosphate synthase (FPS), a central enzyme in cytosolic and mitochondrial isoprenoid biosynthesis, revealed an unexpected role of this pathway in chloroplast development and plastidial isoprenoid metabolism in post-embryonic stages. Upon FPS silencing, chloroplast structure is severely altered, together with a strong reduction in the levels of MEP pathway-derived major end products. This phenotype is associated to misregulation of genes involved in stress responses predominantly belonging to JA and Fe homeostasis pathways. Transcriptomic experiments and analysis of recent literature indicate that sterols are the cause of the observed alterations through an as yet undiscovered mechanism.
Ayudas:	Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad AGL2013-43522-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-1434
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Plant signaling and behavior, Vol. 12, issue 11 (2017) , e1387708, ISSN 1559-2316

DOI: 10.1080/15592324.2017.1387708
PMID: 28990832

5 p, 334.8 KB

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