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Identification and characterization of key circadian clock genes of tobacco hairy roots : putative regulatory role in xenobiotic metabolism
Sosa Alderete, Lucas G. (Universidad Nacional de Río Cuarto. Departamento de Biología Molecular)
Guido, Mario E. (Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Centro de Investigaciones en Química Biológica de Córdoba)
Agostini, Elizabeth (Universidad Nacional de Río Cuarto. Departamento de Biología Molecular)
Mas, Paloma (Centre de Recerca en Agrigenòmica)

Date: 2018
Abstract: The circadian clock is an endogenous system that allows organisms to daily adapt and optimize their physiology and metabolism. We studied the key circadian clock gene (CCG) orthologs in Nicotiana tabacum seedlings and in hairy root cultures (HRC). Putative genes involved in the metabolism of xenobiotic compounds (MXC) were selected and their expression profiles were also analyzed. Seedlings and HRC displayed similar diurnal variations in the expression profiles for the CCG examined under control conditions (CC). MXC-related genes also showed daily fluctuations with specific peaks of expression. However, when HRC were under phenol treatment (PT), the expression patterns of the clock and MXC-related genes were significantly affected. In 2-week-old HRC, PT downregulated the expression of NtLHY, NtTOC1, and NtPRR9 while NtFKF1 and NtGI genes were upregulated by phenol. In 3-week-old HRC, PT also downregulated the expression of all CCG analyzed and NtTOC1 was the most affected. Following PT, the expression of the MXC-related genes was upregulated or displayed an anti-phasic expression profile compared to the expression under CC. Our studies thus provide a glimpse of the circadian expression of clock genes in tobacco and the use of HRC as a convenient system to study plant responses to xenobiotic stresses.
Note: This work was also supported by research grants to P.M. from the Spanish Ministry of Economy and Competitiveness, from the Generalitat de Catalunya (AGAUR), from the Global Research Network of the National Research Foundation of Korea, from the European Commission Marie Curie Research Training Network (ChIP-ET) and from the Spanish Ministry of Economy and Competitiveness, through the “Severo Ochoa Programme for Centres of Excellence in R&D” 2016-2019 (SEV-2015-0533)”.
Note: Número d'acord de subvenció MINECO/SEV-2015-0533
Rights: Tots els drets reservats
Language: Anglès.
Document: article ; recerca ; acceptedVersion
Subject: Tobacco hairy roots ; Circadian clock ; Phenol treatment ; Xenobiotic metabolism
Published in: Environmental science and pollution research, Vol. 25, issue 2 (Jan. 2018) , p. 1597–1608, ISSN 0944-1344

DOI: 10.1007/s11356-017-0579-9

24 p, 597.0 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2017-12-14, last modified 2019-02-04

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