Web of Science: 48 citas, Scopus: 52 citas, Google Scholar: citas
Differential affinity of mammalian histone H1 somatic subtypes for DNA and chromatin
Orrego, Mary (Universidad Autónoma de Barcelona. Departamento de Bioquímica y Biología Molecular)
Ponte Marull, Immaculada (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Roque Córdova, Alicia (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Buschati, Natascha (Universitat Autònoma de Barcelona. Departament de Bioquímica i Biologia Molecular)
Mora Giné, Xavier (Universitat Autònoma de Barcelona. Departament de Matemàtiques)
Suau León, Pere (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Fecha: 2007
Resumen: Histone H1 is involved in the formation and maintenance of chromatin higher order structure. H1 has multiple isoforms; the subtypes differ in timing of expression, extent of phosphorylation and turnover rate. In vertebrates, the amino acid substitution rates differ among subtypes by almost one order of magnitude, suggesting that each subtype might have acquired a unique function. We have devised a competitive assay to estimate the relative binding affinities of histone H1 mammalian somatic subtypes H1a-e and H1° for long chromatin fragments (30–35 nucleosomes) in physiological salt (0. 14 M NaCl) at constant stoichiometry. The H1 complement of native chromatin was perturbed by adding an additional amount of one of the subtypes. A certain amount of SAR (scaffold-associated region) DNA was present in the mixture to avoid precipitation of chromatin by excess H1. SAR DNA also provided a set of reference relative affinities, which were needed to estimate the relative affinities of the subtypes for chromatin from the distribution of the subtypes between the SAR and the chromatin. The amounts of chromatin, SAR and additional H1 were adjusted so as to keep the stoichiometry of perturbed chromatin similar to that of native chromatin. H1 molecules freely exchanged between the chromatin and SAR binding sites. In conditions of free exchange, H1a was the subtype of lowest affinity, H1b and H1c had intermediate affinities and H1d, H1e and H1° the highest affinities. Subtype affinities for chromatin differed by up to 19-fold. The relative affinities of the subtypes for chromatin were equivalent to those estimated for a SAR DNA fragment and a pUC19 fragment of similar length. Avian H5 had an affinity ~12-fold higher than H1e for both DNA and chromatin. H1 subtypes freely exchange in vitro between chromatin binding sites in physiological salt (0. 14 M NaCl). The large differences in relative affinity of the H1 subtypes for chromatin suggest that differential affinity could be functionally relevant and thus contribute to the functional differentiation of the subtypes. The conservation of the relative affinities for SAR and non-SAR DNA, in spite of a strong preference for SAR sequences, indicates that differential affinity alone cannot be responsible for the heterogeneous distribution of some subtypes in cell nuclei.
Nota: Número d'acord de subvenció MEC/BFU2005-02143
Derechos: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Lengua: Anglès.
Documento: article ; recerca ; publishedVersion
Publicado en: BMC biology, Vol. 5 (May 2007) , art. 22, ISSN 1741-7007

PMID: 17498293
DOI: 10.1186/1741-7007-5-22


11 p, 749.2 KB

El registro aparece en las colecciones:
Artículos > Artículos de investigación
Artículos > Artículos publicados

 Registro creado el 2017-12-20, última modificación el 2018-11-14



   Favorit i Compartir