Identification of a type I Ca 2+ /Mg 2+ -dependent endonuclease induced in maize cells exposed to camptothecin
Sánchez-Pons, Nuria (Centre de Recerca en Agrigenòmica)
Vicient Sánchez, Carlos M. (Centre de Recerca en Agrigenòmica)

Date:	2013
Abstract:	Camptothecin is a plant alkaloid that specifically binds topoisomerase I, inhibiting its activity and inducing double stranded breaks in DNA and activating the cell responses to DNA damage. Maize cultured cells were incubated in the presence of different concentrations of camptothecin. Camptothecin inhibits cultured cell growth, induces genomic DNA degradation, and induces a 32 kDa Ca 2+ /Mg 2+ -dependent nuclease activity. This nuclease, we called CaMNUC32, is inhibited by Zn 2+ and by acid pH, it is mainly localized in the nucleus and it cleaves single- and double-stranded DNA, with a higher activity against single-stranded DNA. Two-dimensional electrophoresis combined with mass spectrometry suggests that CaMNUC32 is a member of the type I S1/P1 nuclease family. This type of nucleases are usually Zn 2+ -dependent but our results support previous indications that S1-type nucleases have a wide variety of enzyme activities, including Ca 2+ /Mg 2+ -dependent. We have identified and characterized CaMNUC32, a 32 kDa Ca 2+ /Mg 2+ -dependent nuclease of the S1/P1 family induced by the topoisomerase I inhibitor camptothecin in maize cultured cells.
Grants:	Ministerio de Ciencia y Tecnología AGL2009-09151 Ministerio de Ciencia y Tecnología BIO2004-01577
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Camptothecin ; DNA degradation ; Maize ; Nuclease
Published in:	BMC plant biology, Vol. 13 (Nov. 2013) , art. 186, ISSN 1471-2229

DOI: 10.1186/1471-2229-13-186
PMID: 24256432

9 p, 889.0 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles