Serological diagnosis of canine leishmaniosis : comparison of three commercial ELISA tests (Leiscan ®, ID Screen ® and Leishmania 96 ®), a rapid test (Speed Leish K ®) and an in-house IFAT
Solano Gallego, Laia (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Villanueva-Saz, Sergio
Carbonell, Marta
Trotta, Michele
Furlanello, Tommaso
Natale, Alda

Fecha:	2014
Resumen:	Speed Leish K ® is used as a serological screening test for Leishmania infection prior to vaccination. Limited comparative serological studies with Speed Leish K ® have been performed. The aim of this study was to evaluate the diagnostic performance of four commercially available serologic tests including ELISAs (Leiscan ®, ID Screen ® and Leishmania 96 ®), a rapid test (Speed Leish K ®) and an in-house IFAT for the detection of specific antibodies against Leishmania infantum antigen in dogs in different states of infection. Sick infected dogs (n = 36), healthy infected dogs (n = 18), L. infantum seropositive dogs with low to high levels of antibodies (n = 53), dogs seropositive to other pathogens (to evaluate cross reaction) (n = 14) and uninfected dogs from a non-endemic area (n = 50) and from an endemic area (n = 32) were analysed by the serological methods mentioned above. The sensitivity was as follows: ID Screen ® (0. 953), Leiscan ® and Leishmania 96 ® (0. 925), IFAT (0. 869) and Speed Leish K ® (0. 636). The maximum specificity (1. 000) was attained for all diagnostic tests except the Leishmania 96 ® (0. 896) and IFAT (0. 917). The accuracy was as follows: ID Screen ® (0. 975), Leiscan ® (0. 961), Leishmania 96 ® (0. 911), IFAT (0. 892) and Speed Leish K ® (0. 808). In relation to the area under the ROC curve (AUC-ROC), the maximum value was attained with the ID Screen ® (0. 993) closely followed by Leiscan ® (0. 990), then, Leishmania 96 ® (0. 962), IFAT (0. 926) and Speed Leish K ® (0. 818). For the Kappa index, the best result was obtained by the ID Screen ® (0. 951) followed by Leiscan ® (0. 921), Leishmania 96 ® (0. 822), IFAT (0. 783) and Speed Leish K ® (0. 622). Statistically significant differences were found between the AUC-ROC of quantitative serological tests and the only qualitative rapid test evaluated. There were also statistically significant differences between AUC-ROC of the ELISAs (ID Screen ® and Leiscan ®) and IFAT. Leiscan ® and ID Screen ® had superior diagnostic performance measures than IFAT and all quantitative serological tests were superior when compared to Speed Leish K ®. Thus, Speed Leish K ® may be considered a less valuable screening test prior to vaccination as it may result in vaccination of seropositive dogs and in some cases seropositive sick dogs.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Leishmania infantum ; Dog ; ELISA ; IFAT ; Serological rapid test and vaccine
Publicado en:	Parasites & vectors, Vol. 7 (march 2014) , p. 111, ISSN 1756-3305

DOI: 10.1186/1756-3305-7-111
PMID: 24655335

10 p, 415.1 KB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Grup de Recerca Malalties infeccioses-inflamatòries en animals de companyia (MIAC)
Artículos > Artículos de investigación
Artículos > Artículos publicados