Use of filter papers to determine seroprevalence of Toxoplasma gondii among hunted ungulates in remote Peruvian Amazon
Aston, Emily J. (National Service of Agrarian Health)
Mayor Aparicio, Pedro Ginés (Universitat Autònoma de Barcelona. Departament de Sanitat i d'Anatomia Animals)
Bowman, Dwight (Cornell University College of Veterinary Medicine)
Mohammed, Hussni O. (Cornell University College of Veterinary Medicine)
Liotta, Janice L. (Cornell University College of Veterinary Medicine)
Kwok, Oliver (United States Department of Agriculture, Agriculture Research Service)
Dubey, Jitender P (United States Department of Agriculture, Agriculture Research Service)

Data:	2013
Resum:	Toxoplasmosis is a zoonosis caused by the protozoan Toxoplasma gondii, and it is found worldwide. To determine whether ungulates are reservoirs of T. gondii in an isolated and remote region of the northeastern Peruvian Amazon, antibodies to T. gondii were determined in 5 species of ungulates by the modified agglutination test (MAT). These animals were hunted by subsistence hunters along the Yavarí-Mirín River, in the northeastern Peruvian Amazon. Blood samples were collected by hunters on filter papers. For determination of T. gondii antibodies, blood was eluted from filter papers, and a titer of 1:25 was considered indicative of exposure to T. gondii. Antibodies to T. gondii were found in 26 (31. 0%) peccaries (Pecari tajacu, Tayassu pecari), six (17. 1%) brocket deer (Mazama americana, Mazama gouazoubira), and four (40. 0%) lowland tapir (Tapirus terrestris). We also introduced a modification to the MAT protocol that allows the extraction of fluid samples from several types of laboratory-grade filter paper, thus enabling researchers to easily adapt their approaches to the materials presented to them.
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Toxoplasma gondii ; Modified agglutination test ; Filter paper ; Peccary (Pecari tajacu, Tayassu pecari) ; Brocket deer (Mazama americana, Mazama gouazoubira) ; Lowland tapir (Tapirus terrestris)
Publicat a:	International Journal for Parasitology: Parasites and Wildlife, Vol. 3 (december 2013) , p. 15-19, ISSN 2213-2244

DOI: 10.1016/j.ijppaw.2013.12.001
PMID: 24918073

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