Recombination of the porcine X chromosome : a high density linkage map
Fernández Ávila, Ana Isabel (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya))
Muñoz, María (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya))
Alves, Estefania (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya))
Folch, Josep M. (Centre de Recerca en Agrigenòmica)
Noguera, José Luis (Institut de Recerca i Tecnologia Agroalimentàries)
Perez-Enciso, Miguel (Centre de Recerca en Agrigenòmica)
Rodríguez, Maria del Carmen (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya))
Silió, Luis (Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (Espanya))

Fecha:	2014
Resumen:	Linkage maps are essential tools for the study of several topics in genome biology. High density linkage maps for the porcine autosomes have been constructed exploiting the high density data provided by the PorcineSNP60 BeadChip. However, a high density SSCX linkage map has not been reported up to date. The aim of the current study was to build an accurate linkage map of SSCX to provide precise estimates of recombination rates along this chromosome and creating a new tool for QTL fine mapping. A female-specific high density linkage map was built for SSCX using Sscrofa10. 2 annotation. The total length of this chromosome was 84. 61 cM; although the average recombination rate was 0. 60 cM/Mb, both cold and hot recombination regions were identified. A Bayesian probabilistic to genetic groups and revealed that the animals used in the current study for linkage map construction were likely to be carriers of X chromosomes of European origin. Finally, the newly generated linkage map was used to fine-map a QTL at 16 cM for intramuscular fat content (IMF) measured on longissimus dorsi. The sulfatase isozyme S gene constitutes a functional and positional candidate gene underlying the QTL effect. The current study presents for the first time a high density linkage map for SSCX and supports the presence of cold and hot recombination intervals along this chromosome. The large cold recombination region in the central segment of the chromosome is not likely to be due to structural differences between X chromosomes of European and Asian origin. In addition, the newly generated linkage map has allowed us to fine-map a QTL on SSCX for fat deposition. The online version of this article (doi:10. 1186/s12863-014-0148-x) contains supplementary material, which is available to authorized users.
Ayudas:	Ministerio de Ciencia e Innovación AGL2011-29821-C02 Ministerio de Ciencia e Innovación AGL2008-04818-C03/GAN Ministerio de Ciencia e Innovación CSD2007-00036
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Porcine linkage maps ; Recombination ; X chromosome ; European and Asian X chromosome
Publicado en:	BMC genetics, Vol. 15 (2014) , art. 148, ISSN 1471-2156

DOI: 10.1186/s12863-014-0148-x
PMID: 25526890

6 p, 555.8 KB

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