Web of Science: 17 citations, Scopus: 18 citations, Google Scholar: citations,
Yeast as a heterologous model system to uncover type III effector function
Popa, Crina (Centre de Recerca en Agrigenòmica)
Sánchez Coll, Núria (Centre de Recerca en Agrigenòmica)
Valls, Marc (Centre de Recerca en Agrigenòmica)
Sessa, Guido (Tel Aviv University. Department of Molecular Biology and Ecology of Plants)

Date: 2016
Abstract: Type III effectors (T3E) are key virulence proteins that are injected by bacterial pathogens inside the cells of their host to subvert cellular processes and contribute to disease. The budding yeast Saccharomyces cerevisiae represents an important heterologous system for the functional characterisation of T3E proteins in a eukaryotic environment. Importantly, yeast contains eukaryotic processes with low redundancy and are devoid of immunity mechanisms that counteract T3Es and mask their function. Expression in yeast of effectors from both plant and animal pathogens that perturb conserved cellular processes often resulted in robust phenotypes that were exploited to elucidate effector functions, biochemical properties, and host targets. The genetic tractability of yeast and its amenability for high-throughput functional studies contributed to the success of this system that, in recent years, has been used to study over 100 effectors. Here, we provide a critical view on this body of work and describe advantages and limitations inherent to the use of yeast in T3E research. "Favourite" targets of T3Es in yeast are cytoskeleton components and small GTPases of the Rho family. We describe how mitogen-activated protein kinase (MAPK) signalling, vesicle trafficking, membrane structures, and programmed cell death are also often altered by T3Es in yeast and how this reflects their function in the natural host. We describe how effector structure-function studies and analysis of candidate targeted processes or pathways can be carried out in yeast. We critically analyse technologies that have been used in yeast to assign biochemical functions to T3Es, including transcriptomics and proteomics, as well as suppressor, gain-of-function, or synthetic lethality screens. We also describe how yeast can be used to select for molecules that block T3E function in search of new antibacterial drugs with medical applications. Finally, we provide our opinion on the limitations of S. cerevisiae as a model system and its most promising future applications.
Note: Altres ajuts: grants from the Israel Science Foundation (ISF 326/10) to GS and the COST Action Sustain (FA-1208) supported by COST (European Cooperation in Science and Technology) from the European Union.
Note: Número d'acord de subvenció EC/FP7/331392
Note: Número d'acord de subvenció EC/FP7/321738
Note: Número d'acord de subvenció MINECO/AGL2013-46898-R
Note: Número d'acord de subvenció AGAUR/BP_B 00030
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: article ; recerca ; publishedVersion
Published in: PLoS pathogens, Vol. 12, issue 2 (Feb. 2016) , e1005360, ISSN 1553-7374

DOI: 10.1371/journal.ppat.1005360
PMID: 26914889

17 p, 719.5 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2018-02-07, last modified 2021-02-19

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