Rainbow trout CK9, a CCL25-like ancient chemokine that attracts and regulates B cells and macrophages, the main antigen presenting cells in fish
Aquilino, Carolina (Animal Health Research Center (CISA-INIA))
Granja, Aitor G. (Animal Health Research Center (CISA-INIA))
Castro, Rosario (Animal Health Research Center (CISA-INIA))
Wang, Tiehui (Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen)
Abos, Beatriz (Animal Health Research Center (CISA-INIA))
Parra, David (Universitat Autònoma de Barcelona. Departament de Biologia Cel·lular, de Fisiologia i d'Immunologia)
Secombes, Christopher J. (Scottish Fish Immunology Research Centre, School of Biological Sciences, University of Aberdeen)
Tafalla, Carolina (Animal Health Research Center (CISA-INIA))

Fecha:	2016
Resumen:	CK9 is a rainbow trout (Oncorhynchus mykiss) CC chemokine phylogenetically related to mammalian CCL25. Although CK9 is known to be transcriptionally regulated in response to inflammation particularly in mucosal tissues, its functionality has never been revealed. In the current work, we have demonstrated that CK9 is chemoattractant for antigen presenting cells (APCs) expressing major histocompatibility complex class II (MHC II) on the cell surface. Among these APCs, CK9 has a strong chemotactic capacity for both B cells (IgM + and IgT +) and macrophages. Along with its chemotactic capacities, CK9 modulated the MHC II turnover of B lymphocytes and up-regulated the phagocytic capacity of both IgM + cells and macrophages. Although CK9 had no lymphoproliferative effects, it increased the survival of IgT + lymphocytes. Furthermore, we have established that the chemoattractant capacity of CK9 is strongly increased after pre-incubation of leukocytes with a T-independent antigen, whereas B cell receptor (BCR) cross-linking strongly abrogated their capacity to migrate to CK9, indicating that CK9 preferentially attracts B cells at the steady state or under BCR-independent stimulation. These results point to CK9 being a key regulator of B lymphocyte trafficking in rainbow trout, able to modulate innate functions of teleost B lymphocytes and macrophages.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Materia:	Rainbow trout oncorhynchus mykiss ; Chemokines ; CK9 ; CCL25 ; B cells ; Immunology and Microbiology Section ; Immune response ; Immunity
Publicado en:	Oncotarget, Vol. 7 (march 2016) , p. 17547-17564, ISSN 1949-2553

DOI: 10.18632/oncotarget.8163
PMID: 27003360

18 p, 5.2 MB

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