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A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach
Lopez Girona, Elena (Institut de Recerca i Tecnologia Agroalimentàries)
Zhang, Yu (Centre de Recerca en Agrigenòmica)
Eduardo Muñoz, Iban (Institut de Recerca i Tecnologia Agroalimentàries)
Hernández Mora, José R. (Institut de Recerca i Tecnologia Agroalimentàries)
Alexiou, Konstantinos G. (Institut de Recerca i Tecnologia Agroalimentàries)
Arús i Gorina, Pere (Institut de Recerca i Tecnologia Agroalimentàries)
Aranzana Civit, Mª José (Institut de Recerca i Tecnologia Agroalimentàries)

Date: 2017
Abstract: In peach, the flat phenotype is caused by a partially dominant allele in heterozygosis (Ss), fruits from homozygous trees (SS) abort a few weeks after fruit setting. Previous research has identified a SSR marker (UDP98–412) highly associated with the trait, found suitable for marker assisted selection (MAS). Here we report a ∼10 Kb deletion affecting the gene PRUPE. 6G281100, 400 Kb upstream of UDP98-412, co-segregating with the trait. This gene is a leucine-rich repeat receptor-like kinase (LRRRLK) orthologous to the Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) group. PCR markers suitable for MAS confirmed its strong association with the trait in a collection of 246 cultivars. They were used to evaluate the DNA from a round fruit derived from a somatic mutation of the flat variety 'UFO-4', revealing that the mutation affected the flat associated allele (S). Protein BLAST alignment identified significant hits with genes involved in different biological processes. Best protein hit occurred with AtRLP12, which may functionally complement CLAVATA2, a key regulator that controls the stem cell population size. RT-PCR analysis revealed the absence of transcription of the partially deleted allele. The data support PRUPE. 6G281100 as a candidate gene for flat shape in peach.
Note: Número d'acord de subvenció MINECO/AGL2015-68329-R
Note: Número d'acord de subvenció MINECO/SEV-2015-0533
Note: Altres ajuts: CERCA Programme/Generalitat de Catalunya
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Published in: Scientific reports, Vol. 7, (July 2017) , art. 6714, ISSN 2045-2322

DOI: 10.1038/s41598-017-07022-0
PMID: 28751691


11 p, 2.5 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2018-02-07, last modified 2019-07-21



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