A deletion affecting an LRR-RLK gene co-segregates with the fruit flat shape trait in peach
López-Girona, Elena (Institut de Recerca i Tecnologia Agroalimentàries)
Zhang, Yu (Centre de Recerca en Agrigenòmica)
Eduardo, Iban (Institut de Recerca i Tecnologia Agroalimentàries)
Hernández Mora, José R. (Institut de Recerca i Tecnologia Agroalimentàries)
Alexiou, Konstantinos G. (Institut de Recerca i Tecnologia Agroalimentàries)
Arús i Gorina, Pere (Institut de Recerca i Tecnologia Agroalimentàries)
Aranzana, Maria José (Institut de Recerca i Tecnologia Agroalimentàries)

Date:	2017
Abstract:	In peach, the flat phenotype is caused by a partially dominant allele in heterozygosis (Ss), fruits from homozygous trees (SS) abort a few weeks after fruit setting. Previous research has identified a SSR marker (UDP98-412) highly associated with the trait, found suitable for marker assisted selection (MAS). Here we report a ∼10 Kb deletion affecting the gene PRUPE. 6G281100, 400 Kb upstream of UDP98-412, co-segregating with the trait. This gene is a leucine-rich repeat receptor-like kinase (LRRRLK) orthologous to the Brassinosteroid insensitive 1-associated receptor kinase 1 (BAK1) group. PCR markers suitable for MAS confirmed its strong association with the trait in a collection of 246 cultivars. They were used to evaluate the DNA from a round fruit derived from a somatic mutation of the flat variety 'UFO-4', revealing that the mutation affected the flat associated allele (S). Protein BLAST alignment identified significant hits with genes involved in different biological processes. Best protein hit occurred with AtRLP12, which may functionally complement CLAVATA2, a key regulator that controls the stem cell population size. RT-PCR analysis revealed the absence of transcription of the partially deleted allele. The data support PRUPE. 6G281100 as a candidate gene for flat shape in peach.
Grants:	Ministerio de Economía y Competitividad AGL2015-68329-R Ministerio de Economía y Competitividad SEV-2015-0533
Note:	Altres ajuts: CERCA Programme/Generalitat de Catalunya
Note:	Aquest article té una correcció a 10.1038/s41598-022-09334-2
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	Scientific reports, Vol. 7 (July 2017) , art. 6714, ISSN 2045-2322

Correcció de l'article: https://ddd.uab.cat/record/258132
DOI: 10.1038/s41598-017-07022-0
PMID: 28751691

11 p, 2.5 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles