Web of Science: 10 citations, Scopus: 9 citations, Google Scholar: citations,
Hedgehog Pathway Inhibition Hampers Sphere and Holoclone Formation in Rhabdomyosarcoma
Almazán-Moga, Anna (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Zarzosa, Patricia (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Vidal, Isaac (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Molist, Carla (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Giralt, Irina (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Navarro Barea, Natalia (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Soriano, Aroa (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Segura, Miguel F. (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Alfranca, Arantza (Instituto de Salud Carlos III)
Garcia-Castro, Javier (Instituto de Salud Carlos III)
Sánchez de Toledo, José (Universitat Autònoma de Barcelona. Departament de Pediatria, Obstetrícia i Ginecologia i Medicina Preventiva i Salut Pública)
Roma, Josep (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Gallego, Soledad (Universitat Autònoma de Barcelona. Departament de Pediatria, Obstetrícia i Ginecologia i Medicina Preventiva i Salut Pública)

Date: 2017
Abstract: Rhabdomyosarcoma (RMS) is the most common type of soft tissue sarcoma in children and can be divided into two main subtypes: embryonal (eRMS) and alveolar (aRMS). Among the cellular heterogeneity of tumors, the existence of a small fraction of cells called cancer stem cells (CSC), thought to be responsible for the onset and propagation of cancer, has been demonstrated in some neoplasia. Although the existence of CSC has been reported for eRMS, their existence in aRMS, the most malignant subtype, has not been demonstrated to date. Given the lack of suitable markers to identify this subpopulation in aRMS, we used cancer stem cell-enriched supracellular structures (spheres and holoclones) to study this subpopulation. This strategy allowed us to demonstrate the capacity of both aRMS and eRMS cells to form these structures and retain self-renewal capacity. Furthermore, cells contained in spheres and holoclones showed significant Hedgehog pathway induction, the inhibition of which (pharmacologic or genetic) impairs the formation of both holoclones and spheres. Our findings point to a crucial role of this pathway in the maintenance of these structures and suggest that Hedgehog pathway targeting in CSC may have great potential in preventing local relapses and metastases.
Grants: Instituto de Salud Carlos III RTICC-RD12-0036-0016
Instituto de Salud Carlos III RTICC-RD12-0036-0027
Instituto de Salud Carlos III PI11-00740
Instituto de Salud Carlos III PI14-00647
Note: Altres ajuts: This work was supported by grants from Institut Català d'Oncologia (ICO), Instituto de Salud Carlos III (RTICC-RD12/0036/0016 and RD12/0036/0027; PI11/00740 and PI14/00647), Fundació A. BOSCH, and ajuts predoctorals VHIR.
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: Article ; recerca ; Versió publicada
Subject: Hedgehog ; Rhabdomyosarcoma
Published in: Stem Cells International, Vol. 2017 (january 2017) , ISSN 1687-9678

DOI: 10.1155/2017/7507380
PMID: 28243259


14 p, 3.4 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2018-02-08, last modified 2023-09-27



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