Focused transhepatic electroporation mediated by hypersaline infusion through the portal vein in rat model : preliminary results on differential conductivity
Pañella, Clara (Institut Hospital del Mar d'Investigacions Mèdiques)
Castellví, Quim (Universitat Pompeu Fabra. Departament de Tecnologies de la Informació i les Comunicacions)
Moll, Xavier (Institut Hospital del Mar d'Investigacions Mèdiques)
Quesada, Rita (Institut Hospital del Mar d'Investigacions Mèdiques)
Villanueva, Alberto (Institut Català d'Oncologia)
Iglesias, Mar (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Naranjo-Hans, Dolores (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Sánchez-Velázquez, Patricia (Institut Hospital del Mar d'Investigacions Mèdiques)
Andaluz Martínez, Anna (Institut Hospital del Mar d'Investigacions Mèdiques)
Grande, L (Institut Hospital del Mar d'Investigacions Mèdiques)
Ivorra, Antoni (Universitat Pompeu Fabra. Departament de Tecnologies de la Informació i les Comunicacions)
Burdío, Fernando (Institut Hospital del Mar d'Investigacions Mèdiques)

Date:	2017
Abstract:	Spread hepatic tumours are not suitable for treatment either by surgery or conventional ablation methods. The aim of this study was to evaluate feasibility and safety of selectively increasing the healthy hepatic conductivity by the hypersaline infusion (HI) through the portal vein. We hypothesize this will allow simultaneous safe treatment of all nodules by irreversible electroporation (IRE) when applied in a transhepatic fashion. Sprague Dawley (Group A, n = 10) and Athymic rats with implanted hepatic tumour (Group B, n = 8) were employed. HI was performed (NaCl 20%, 3. 8 mL/Kg) by trans-splenic puncture. Deionized serum (40 mL/Kg) and furosemide (2 mL/Kg) were simultaneously infused through the jugular vein to compensate hypernatremia. Changes in conductivity were monitored in the hepatic and tumour tissue. The period in which hepatic conductivity was higher than tumour conductivity was defined as the therapeutic window (TW). Animals were monitored during 1-month follow-up. The animals were sacrificed and selective samples were used for histological analysis. The overall survival rate was 82. 4% after the HI protocol. The mean maximum hepatic conductivity after HI was 2. 7 and 3. 5 times higher than the baseline value, in group A and B, respectively. The mean maximum hepatic conductivity after HI was 1. 4 times higher than tumour tissue in group B creating a TW to implement selective IRE. HI through the portal vein is safe when the hypersaline overload is compensated with deionized serum and it may provide a TW for focused IRE treatment on tumour nodules.
Grants:	Ministerio de Economía y Competitividad TEC2014-52383-C3-R
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Irreversible electroporation ; Liver tumour ; Electrical conductivity
Published in:	Radiology and oncology, Vol. 51, issue 4 (2017) , p. 415-421, ISSN 1581-3207

DOI: 10.1515/raon-2017-0051
PMID: 29333120

7 p, 828.5 KB

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