Analysis of hepatitis B virus preS1 variability and prevalence of the rs2296651 polymorphism in a Spanish population
Casillas, Rosario (Hospital Universitari Vall d'Hebron)
Tabernero, David (Hospital Universitari Vall d'Hebron)
Gregori i Font, Josep (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Belmonte, Irene (Hospital Universitari Vall d'Hebron)
Cortese, Maria Francesca (Hospital Universitari Vall d'Hebron)
González, Carolina (Hospital Universitari Vall d'Hebron)
Riveiro Barciela, Mar
López, Rosa Maria (Hospital Universitari Vall d'Hebron)
Quer, Josep 1965- (Hospital Universitari Vall d'Hebron. Institut de Recerca)
Esteban, Rafael
Buti, Maria
Rodríguez Frías, Francisco (Hospital Universitari Vall d'Hebron)
Universitat Autònoma de Barcelona

Fecha:	2018
Resumen:	To determine the variability/conservation of the domain of hepatitis B virus (HBV) preS1 region that interacts with sodium-taurocholate cotransporting polypeptide (hereafter, NTCP-interacting domain) and the prevalence of the rs2296651 polymorphism (S267F, NTCP variant) in a Spanish population. Serum samples from 246 individuals were included and divided into 3 groups: patients with chronic HBV infection (CHB) (n = 41, 73% Caucasians), patients with resolved HBV infection (n = 100, 100% Caucasians) and an HBV-uninfected control group (n = 105, 100% Caucasians). Variability/conservation of the amino acid (aa) sequences of the NTCP-interacting domain, (aa 2-48 in viral genotype D) and a highly conserved preS1 domain associated with virion morphogenesis (aa 92-103 in viral genotype D) were analyzed by next-generation sequencing and compared in 18 CHB patients with viremia.
Ayudas:	Ministerio de Economía y Competitividad PI14/01416 Ministerio de Economía y Competitividad PI15/00856
Nota:	Altres ajuts: Cofinanced by the European Regional Development Fund (ERDF); and the Gilead Fellowship Program, No. GLD14-00296.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Documento:	Article
Materia:	Hepatitis B virus ; Hepatitis B virus preS1 region ; Sodium-taurocholate co-transporting polypeptide ; NTCP-interacting domain ; Virion morphogenesis domain ; SNP rs2296651 ; Next-generation sequencing ; Real-time PCR melting curves
Publicado en:	World Journal of Gastroenterology, Vol. 24 (february 2018), p. 680-692, ISSN 2219-2840

14 p, 1.3 MB

El registro aparece en las colecciones:
Artículos > Artículos de investigación
Artículos > Artículos publicados