Sensitive and label-free detection of miRNA-145 by triplex formation
Aviñó, Ana (Institut de Química Avançada de Catalunya)
Huertas, César S. (Institut Català de Nanociència i Nanotecnologia)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)
Eritja, Ramon (Institut de Química Avançada de Catalunya)

Date:	2016
Abstract:	The development of new strategies for detecting microRNAs (miRNAs) has become a crucial step in the diagnostic field. miRNA profiles depend greatly on the sample and the analytical platform employed, leading sometimes to contradictory results. In this work, we study the use of modified parallel tail-clamps to detect a miRNA sequence involved in tumor suppression by triplex formation. Thermal denaturing curves and circular dichroism (CD) measurements have been performed to confirm that parallel clamps carrying 8-aminoguanine form the most stable triplex structures with their target miRNA. The modified tail-clamps have been tested as bioreceptors in a surface plasmon resonance (SPR) biosensor for the detection of miRNA-145. The detection limit was improved 2. 4 times demonstrating that a stable triplex structure is formed between target miRNA and 8-aminoguanine tail-clamp bioreceptor. This new approach is an essential step toward the label-free and reliable detection of miRNA signatures for diagnostic purposes.
Grants:	Ministerio de Economía y Competitividad CTQ2010-20541 Ministerio de Economía y Competitividad CTQ2014-52588-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2009/SGR-208 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-624
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	8-Amino-2'-deoxyguanosine ; Parallel tail-clamp ; SPR biosensor ; Target miRNA-145 ; Triplex
Published in:	Analytical and bioanalytical chemistry, Vol. 408, Issue 3 (January 2016) , p. 885-893, ISSN 1618-2650

DOI: 10.1007/s00216-015-9180-6

Post-print 17 p, 782.3 KB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles