Circadian waves of transcriptional repression shape PIF-regulated photoperiod-responsive growth in Arabidopsis
Martin, Guiomar (Centre de Recerca en Agrigenòmica)
Rovira, Arnau (Centre de Recerca en Agrigenòmica)
Veciana, Nil (Centre de Recerca en Agrigenòmica)
Soy, Judit (Centre de Recerca en Agrigenòmica)
Toledo-Ortiz, Gabriela (Lancaster Environment Center)
Gommers, Charlotte M. M. (Centre de Recerca en Agrigenòmica)
Boix, Marc (Centre de Recerca en Agrigenòmica)
Henriques, Rossana (Centre de Recerca en Agrigenòmica)
Minguet, Eugenio G. (Instituto de Biología Molecular y Celular de Plantas)
Alabadí, David (Instituto de Biología Molecular y Celular de Plantas)
Halliday, Karen J. (The University of Edinburgh)
Leivar, Pablo (Centre de Recerca en Agrigenòmica)
Monte Collado, Elena (Centre de Recerca en Agrigenòmica)

Fecha:	2018
Resumen:	Plants coordinate their growth and development with the environment through integration of circadian clock and photosensory pathways. In Arabidopsis thaliana, rhythmic hypocotyl elongation in short days (SD) is enhanced at dawn by the basic-helix-loop-helix (bHLH) transcription factors PHYTOCHROME-INTERACTING FACTORS (PIFs) directly inducing expression of growth-related genes [1-6]. PIFs accumulate progressively during the night and are targeted for degradation by active phytochromes in the light, when growth is reduced. Although PIF proteins are also detected during the day hours [7-10], their growth-promoting activity is inhibited through unknown mechanisms. Recently, the core clock components and transcriptional repressors PSEUDO-RESPONSE REGULATORS PRR9/7/5 [11, 12], negative regulators of hypocotyl elongation [13, 14], were described to associate to G boxes [15], the DNA motifs recognized by the PIFs [16, 17], suggesting that PRR and PIF function might converge antagonistically to regulate growth. Here we report that PRR9/7/5 and PIFs physically interact and bind to the same promoter region of pre-dawn-phased, growth-related genes, and we identify the transcription factor CDF5 [18, 19] as target of this interplay. In SD, CDF5 expression is sequentially repressed from morning to dusk by PRRs and induced pre-dawn by PIFs. Consequently, CDF5 accumulates specifically at dawn, when it induces cell elongation. Our findings provide a framework for recent TIMING OF CAB EXPRESSION 1 (TOC1/PRR1) data [5, 20] and reveal that the long described circadian morning-to-midnight waves of the PRR transcriptional repressors (PRR9, PRR7, PRR5, and TOC1) [21] jointly gate PIF activity to dawn to prevent overgrowth through sequential regulation of common PIF-PRR target genes such as CDF5.
Ayudas:	European Commission 334052 European Commission 256420 Ministerio de Economía y Competitividad BIO2015-68460-P Ministerio de Economía y Competitividad BIO2015-70812-ERC Ministerio de Economía y Competitividad SEV-2015-0533 Ministerio de Economía y Competitividad AGL2014-57200-JIN Ministerio de Economía y Competitividad BIO2013-43184-P Ministerio de Economía y Competitividad BIO2012-31672 Ministerio de Economía y Competitividad RYC-2011-09220 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-1406
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Plant diurnal growth ; Hypocotyl elongation ; Gating of plant responses ; Phytochrome-interacting factors PIFs ; Circadian clock ; Pseudo-response regulators PRRs ; TOC1 ; Cycling DOF factor CDF5 ; Transcriptional regulation ; Arabidopsis
Publicado en:	Current biology, Vol. 28, issue 2 (Jan. 2018) , p. 311-318, ISSN 0960-9822

DOI: 10.1016/j.cub.2017.12.021
PMID: 29337078

Post-print 73 p, 4.0 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > CRAG (Centro de Investigación en Agrigenómica)
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