Web of Science: 7 citations, Scopus: 6 citations, Google Scholar: citations,
Circadian waves of transcriptional repression shape PIF-regulated photoperiod-responsive growth in Arabidopsis
Martin, Guiomar (Centre de Recerca en Agrigenòmica)
Rovira, Arnau (Centre de Recerca en Agrigenòmica)
Veciana, Nil (Centre de Recerca en Agrigenòmica)
Soy, Judit (Centre de Recerca en Agrigenòmica)
Toledo-Ortiz, Gabriela (Lancaster Environment Center)
Gommers, Charlotte M.M. (Centre de Recerca en Agrigenòmica)
Boix, Marc (Centre de Recerca en Agrigenòmica)
Henriques, Rossana (Centre de Recerca en Agrigenòmica)
Minguet, Eugenio G. (Universitat Politècnia de València. Instituto de Biología Molecular y Celular de Plantas)
Alabadí, David (Universitat Politècnia de València. Instituto de Biología Molecular y Celular de Plantas)
Halliday, Karen J. (The University of Edinburgh)
Leivar, Pablo (Centre de Recerca en Agrigenòmica)
Monte Collado, Elena (Centre de Recerca en Agrigenòmica)

Date: 2018
Abstract: Plants coordinate their growth and development with the environment through integration of circadian clock and photosensory pathways. In Arabidopsis thaliana, rhythmic hypocotyl elongation in short days (SD) is enhanced at dawn by the basic-helix-loop-helix (bHLH) transcription factors PHYTOCHROME-INTERACTING FACTORS (PIFs) directly inducing expression of growth-related genes [1-6]. PIFs accumulate progressively during the night and are targeted for degradation by active phytochromes in the light, when growth is reduced. Although PIF proteins are also detected during the day hours [7-10], their growth-promoting activity is inhibited through unknown mechanisms. Recently, the core clock components and transcriptional repressors PSEUDO-RESPONSE REGULATORS PRR9/7/5 [11, 12], negative regulators of hypocotyl elongation [13, 14], were described to associate to G boxes [15], the DNA motifs recognized by the PIFs [16, 17], suggesting that PRR and PIF function might converge antagonistically to regulate growth. Here we report that PRR9/7/5 and PIFs physically interact and bind to the same promoter region of pre-dawn-phased, growth-related genes, and we identify the transcription factor CDF5 [18, 19] as target of this interplay. In SD, CDF5 expression is sequentially repressed from morning to dusk by PRRs and induced pre-dawn by PIFs. Consequently, CDF5 accumulates specifically at dawn, when it induces cell elongation. Our findings provide a framework for recent TIMING OF CAB EXPRESSION 1 (TOC1/PRR1) data [5, 20] and reveal that the long described circadian morning-to-midnight waves of the PRR transcriptional repressors (PRR9, PRR7, PRR5, and TOC1) [21] jointly gate PIF activity to dawn to prevent overgrowth through sequential regulation of common PIF-PRR target genes such as CDF5.
Note: Número d'acord de subvenció EC/FP7/334052
Note: Número d'acord de subvenció EC/FP7/256420
Note: Número d'acord de subvenció MINECO/BIO2015-68460-P
Note: Número d'acord de subvenció MINECO/BIO2015-70812-ERC
Note: Número d'acord de subvenció MINECO/SEV-2015-0533
Note: Número d'acord de subvenció MINECO/AGL2014-57200-JIN
Note: Número d'acord de subvenció MINECO/BIO2013-43184-P
Note: Número d'acord de subvenció MINECO/BIO2012-31672
Note: Número d'acord de subvenció MINECO/RYC-2011-09220
Note: Número d'acord de subvenció AGAUR/2014/SGR-1406
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Language: Anglès.
Document: article ; recerca ; acceptedVersion
Subject: Plant diurnal growth ; Hypocotyl elongation ; Gating of plant responses ; Phytochrome-interacting factors PIFs ; Circadian clock ; Pseudo-response regulators PRRs ; TOC1 ; Cycling DOF factor CDF5 ; Transcriptional regulation ; Arabidopsis
Published in: Current biology, Vol. 28, issue 2 (Jan. 2018) , p. 311-318, ISSN 0960-9822

DOI: 10.1016/j.cub.2017.12.021

73 p, 4.0 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles

 Record created 2018-09-18, last modified 2019-02-04

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