Dual carbon-chlorine isotope fractionation during dichloroelimination od 1,1,2-trichloroethane by an enrichment culture containing Dehalogenimonas sp
Rosell, Mònica (Universitat de Barcelona. Departament de Mineralogia, Petrologia i Geologia Aplicada)
Palau Capdevila, Jordi (Universitat de Barcelona. Departament de Mineralogia, Petrologia i Geologia Aplicada)
Mortan, Siti Hatijah (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)
Caminal i Saperas, Glòria (Institut de Química Avançada de Catalunya)
Soler, Albert (Universitat de Barcelona. Departament de Mineralogia, Petrologia i Geologia Aplicada)
Shouakar Stash, Orfan (University of Waterloo. Department of Earth and Environmental Sciences)
Marco Urrea, Ernest (Universitat Autònoma de Barcelona. Departament d'Enginyeria Química, Biològica i Ambiental)

Date:	2019
Abstract:	Chlorinated ethanes are frequent groundwater contaminants but compound specific isotope analysis (CSIA) has been scarcely applied to investigate their degradation pathways. In this study, dual carbon and chlorine isotope fractionation was used to investigate for the first time the anoxic biodegradation of 1,1,2-trichloroethane (1,1,2-TCA) using a Dehalogenimonas-containing culture. The isotopic fractionation values obtained for the biodegradation of 1,1,2-TCA were ɛC = −6. 9 ± 0. 4‰ and ɛCl = −2. 7 ± 0. 3‰. The detection of vinyl chloride (VC) as unique byproduct and a closed carbon isotopic mass balance corroborated that dichloroelimination was the degradation pathway used by this strain. Combining the values of δ13C and δ37Cl resulted in a dual element C-Cl isotope slope of Λ = 2. 5 ± 0. 2‰. Investigation of the apparent kinetic isotope effects (AKIEs) expected for cleavage of a CCl bond showed an important masking of the intrinsic isotope fractionation. Theoretical calculation of Λ suggested that dichloroelimination of 1,1,2-TCA was taking place via simultaneous cleavage of two CCl bonds (concerted reaction mechanism). The isotope data obtained in this study can be useful to monitor natural attenuation of 1,1,2-TCA via dichloroelimination and provide insights into the source and fate of VC in contaminated groundwaters.
Grants:	Ministerio de Economía y Competitividad CTM2013-48545-C2-1-R Ministerio de Economía y Competitividad CTM2016-75587-C2-1-R Ministerio de Economía y Competitividad CGL2014-57215-C4-1-R Ministerio de Economía y Competitividad CGL2017-87216-C4-1-R Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-14 Agència de Gestió d'Ajuts Universitaris i de Recerca 2017/SGR-1733 Ministerio de Economía y Competitividad RYC-2012-11920
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Language:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Subject:	Dehalogenimonas ; Dual isotope fractionation ; Dichloroelimination ; Organohalide-respiring bacteria ; 1,1,2-Trichloroethane
Published in:	Science of the total environment, Vol. 648, (January 2019) , p. 422-429, ISSN 1879-1026

DOI: 10.1016/j.scitotenv.2018.08.071
PMID: 30121041

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