Electromechanical Conditioning of Adult Progenitor Cells Improves Recovery of Cardiac Function After Myocardial Infarction
Llucià-Valldeperas, Aida (Institut Germans Trias i Pujol. Hospital Universitari Germans Trias i Pujol)
Soler Botija, Carolina (Institut Germans Trias i Pujol)
Gálvez-Montón, Carolina (Institut Germans Trias i Pujol)
Rudilla, F. (Institut Germans Trias i Pujol)
Prat-Vidal, Cristina (Institut Germans Trias i Pujol)
Perea Gil, Isaac (Institut Germans Trias i Pujol)
Sanchez, Benjamin (Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica)
Bragos, Ramon (Universitat Politècnica de Catalunya. Departament d'Enginyeria Electrònica)
Vunjak-Novakovic, Gordana (Columbia University. Biomedical Engineering and Medicine)
Bayés-Genís, Antoni (Universitat Autònoma de Barcelona. Departament de Medicina)

Fecha:	2017
Resumen:	Cardiac cells are subjected to mechanical and electrical forces, which regulate gene expression and cellular function. Therefore, in vitro electromechanical stimuli could benefit further integration of therapeutic cells into the myocardium. Our goals were (a) to study the fiability of a tissueengineered construct with cardiac adipose tissue-derived progenitor cells (cardiac ATDPCs) and (b) to examine the effect of electromechanically stimulated cardiac ATDPCs within a myocardial infarction (MI) model in mice for the first time. Cardiac ATDPCs were electromechanically stimulated at 2-millisecond pulses of 50 mV/cm at 1 Hz and 10% stretching during 7 days. The cells were harvested, labeled, embedded in a fibrin hydrogel, and implanted over the infarcted area of the murine heart. A total of 39 animals were randomly distributed and sacrificed at 21 days: groups of grafts without cells and with stimulated or nonstimulated cells. Echocardiography and gene and protein analyses were also carried out. Physiologically stimulated ATDPCs showed increased expression of cardiac transcription factors, structural genes, and calcium handling genes. At 21 days after implantation, cardiac function (measured as left ventricle ejection fraction between presacrifice and post-MI) increased up to 12% in stimulated grafts relative to nontreated animals. Vascularization and integration with the host blood supply of grafts with stimulated cells resulted in increased vessel density in the infarct border region. Trained cells within the implanted fibrin patch expressed main cardiac markers and migrated into the underlying ischemic myocardium. To conclude, synchronous electromechanical cell conditioning before delivery may be a preferred alternative when considering strategies for heart repair after myocardial infarction.
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Stem cells translational medicine, Vol. 6 (2017) , p. 970-981

DOI: 10.5966/sctm.2016-0079
PMID: 28297585

12 p, 3.6 MB

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Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias de la salud y biociencias > Institut d'Investigació en Ciencies de la Salut Germans Trias i Pujol (IGTP)
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