Unveiling the multifaceted mechanisms of antibacterial activity of buforin II and frenatin 2.3S peptides from skin micro-organs of the orinoco lime treefrog (Sphaenorhynchus lacteus)
Muñoz-Camargo, Carolina (Universidad de los Andes)
Salazar Montoya, Vivian Angélica (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Barrero-Guevara, Laura (Universidad de los Andes)
Camargo, Sandra (Universidad de los Andes)
Mosquera, Ángela (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Groot, Helena (Universidad de los Andes)
Boix i Borràs, Esther (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)

Data:	2018
Resum:	Amphibian skin is a rich source of natural compounds with diverse antimicrobial and immune defense properties. Our previous studies showed that the frog skin secretions obtained by skin micro-organs from various species of Colombian anurans have antimicrobial activities against bacteria and viruses. We purified for the first time two antimicrobial peptides from the skin micro-organs of the Orinoco lime treefrog (Sphaenorhynchus lacteus) that correspond to Buforin II (BF2) and Frenatin 2. 3S (F2. 3S). Here, we have synthesized the two peptides and tested them against Gram-negative and Gram-positive bacteria, observing an effective bactericidal activity at micromolar concentrations. Evaluation of BF2 and F2. 3S membrane destabilization activity on bacterial cell cultures and synthetic lipid bilayers reveals a distinct membrane interaction mechanism. BF2 agglutinates E. coli cells and synthetic vesicles, whereas F2. 3S shows a high depolarization and membrane destabilization activities. Interestingly, we found that F2. 3S is able to internalize within bacterial cells and can bind nucleic acids, as previously reported for BF2. Moreover, bacterial exposure to both peptides alters the expression profile of genes related to stress and resistance response. Overall, these results show the multifaceted mechanism of action of both antimicrobial peptides that can provide alternative tools in the fight against bacterial resistance.
Ajuts:	Ministerio de Economía y Competitividad SAF2015-66007P Ministerio de Economía y Competitividad BFU2012-38695
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Llengua:	Anglès
Document:	Article ; recerca ; Versió publicada
Matèria:	Antibacterial activity ; Antimicrobial peptides ; Bacterial agglutination ; Buforin II ; DNA binding ; Frenatin 2.3S ; Frog skin secretions ; Membrane translocation ; Membrane leakage
Publicat a:	International journal of molecular sciences, Vol. 19, issue 8 (August 2018) , art. 2170, ISSN 1422-0067

DOI: 10.3390/ijms19082170
PMID: 30044391

21 p, 2.5 MB

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