Alzheimer's disease biomarkers detection in human samples by efficient capturing through porous magnetic microspheres and labelling with electrocatalytic gold nanoparticles
De La Escosura-Muñiz, Alfredo (Institut Català de Nanociència i Nanotecnologia)
Plichta, Zdeněk (Akademie věd České republiky)
Horák, Daniel (Akademie věd České republiky)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Fecha:	2015
Resumen:	A nanobiosensor based on the use of porous magnetic microspheres (PMM) as efficient capturing/pre-concentrating platform is presented for detection of Alzheimer's disease (AD) biomarkers. These PMMs prepared by a multistep swelling polymerization combined with iron oxide precipitation afford carboxyl functional groups suitable for immobilization of antibodies on the particle surface allowing an enhanced efficiency in the capturing of AD biomarkers from human serum samples. The AD biomarkers signaling is produced by gold nanoparticle (AuNP) tags monitored through their electrocatalytic effect towards hydrogen evolution reaction (HER). Novel properties of PMMs in terms of high functionality and high active area available for enhanced catalytic activity of the captured AuNPs electrocatalytic tags are exploited for the first time. A thorough characterization by scanning transmission electron microscope in high angle annular dark field mode (STEM-HAADF) demonstrates the enhanced ability of PMMs to capture a higher quantity of analyte and consequently of electrocatalytic label, when compared with commercially available microspheres. The optimized and characterized PMMs are also applied for the first time for the detection of beta amyloid and ApoE at clinical relevant levels in cerebrospinal fluid (CSF), serum and plasma samples of patients suffering from AD.
Ayudas:	European Commission 246513 Ministerio de Economía y Competitividad MAT2011-25870
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Alzheimer disease biomarker ; Electrochemical immunoassay ; Gold nanoparticles ; Hydrogen evolution reaction ; Porous magnetic microspheres
Publicado en:	Biosensors & bioelectronics, Vol. 67 (May 2015) , p. 162-169, ISSN 1873-4235

DOI: 10.1016/j.bios.2014.07.086

Postprint 32 p, 913.8 KB

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