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Supplementing maturation medium with insulin-like growth factor I and vitrification medium with reduced glutathione enhances survival rates and development ability of 'in vitro' matured, vitrified-warmed pig oocytes
Azevedo Pereira, Bárbara (Universitat de Girona. Institut de Tecnologia Agroalimentària)
Zangeronimo, Marcio Gilberto (Federal University of Lavras, Brazil. Department of Veterinary Medicine)
Castillo-Martín, Míriam (Universitat de Girona. Institut de Tecnologia Agroalimentària)
Gadani, Beatrice (Universitat de Girona. Institut de Tecnologia Agroalimentària)
Resende Chaves, Bruna (Universitat de Girona. Institut de Tecnologia Agroalimentària)
Rodríguez-Gil, Joan-Enric (Universitat Autònoma de Barcelona. Departament de Medicina i Cirurgia Animals)
Bonet, Sergi (Universitat de Girona. Institut de Tecnologia Agroalimentària)
Yeste, Marc (Universitat de Girona. Institut de Tecnologia Agroalimentària)

Date: 2018
Abstract: The present study sought to determine whether in vitro maturation (IVM) of pig oocytes in a medium supplemented with insulin growth factor-I (IGF-I) and subsequent vitrification with or without reduced glutathione (GSH) affect their quality and developmental competence, and the expression of genes involved in antioxidant, apoptotic and stress responses. In Experiment 1, cumulus-oocyte complexes were matured in the absence or presence of IGF-I (100 ng·mL−1) and then vitrified-warmed with or without 2mM of GSH. Maturation rate was evaluated before vitrification, and oocyte viability, DNA fragmentation and relative transcript abundances of BCL-2-associated X protein (BAX), BCL2-like1 (BCL2L1), heat shock protein 70 (HSPA1A), glutathione peroxidase 1 (GPX1) and superoxide dismutase 1 (SOD1) genes were assessed in fresh and vitrified-warmed oocytes. In Experiment 2, fresh and vitrified-warmed oocytes were in vitro fertilized and their developmental competence determined. Whereas the addition of IGF-I to maturation medium had no effect on oocyte maturation, it caused an increase in the survival rate of vitrified-warmed oocytes. This effect was accompanied by a concomitant augment in the relative transcript abundance of HSPA1A and a decrease of BAX. Furthermore, the addition of GSH to vitrification-warming media increased survival rates at post-warming. Likewise, the action of GSH was concomitant with an increase in the relative abundance of GPX1 and a decrease of BAX transcript. Blastocyst rates of vitrified-warmed oocytes did not differ from their fresh counterparts when IGF-I and GSH were combined. In conclusion, supplementing maturation medium with 100 ng·mL−1 IGF-I and vitrification-warming solutions with 2mM GSH improves the quality and cryotolerance of IVM pig oocytes, through a mechanism that involves BAX, GPX1 and HSPA1A expression.
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Growth factors ; IGF-I ; Antioxidants ; GSH ; Apoptosis ; Cryotolerance ; DNA fragmentation ; Swine
Published in: Frontiers in Vascular Physiology, 2018 , ISSN 1664-042X

Adreça alternativa: https://scholar.google.cat/scholar?q=Pereira+Supplementing+maturation+medium+with+insulin-like
DOI: 10.3389/fphys.2018.01894
PMID: 30692931

13 p, 1.6 MB

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Articles > Research articles
Articles > Published articles

 Record created 2019-01-18, last modified 2019-02-12

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