Web of Science: 7 cites, Scopus: 6 cites, Google Scholar: cites,
Assessment of BCG and inactivated Mycobacterium bovis vaccines in an experimental tuberculosis infection model in sheep
Balseiro, Ana (SERIDA. Servicio Regional de Investigación y Desarrollo Agroalimentario. Centro de Biotecnología Animal)
Altuzarra, Raúl (Universitat Autònoma de Barcelona. Departament de Medicina i Cirugia Animals)
Vidal Barba, Enric (Universitat Autònoma de Barcelona. Centre de Recerca en Sanitat Animal)
Moll Sánchez, Xavier (Universitat Autònoma de Barcelona. Departament de Medicina i Cirugia Animals)
Espada Gerlach, Yvonne (Universitat Autònoma de Barcelona. Departament de Medicina i Cirugia Animals)
Sevilla, Iker A. (NEIKER-Tecnalia. Instituto Vasco de Investigación y Desarrollo Agrario. Departamento de Sanidad Animal)
Domingo Álvarez, Mariano (Universitat Autònoma de Barcelona. Departament de Medicina i Cirugia Animals)
Garrido, Joseba M. (NEIKER-Tecnalia. Instituto Vasco de Investigación y Desarrollo Agrario. Departamento de Sanidad Animal)
Juste, Ramón A. (SERIDA. Servicio Regional de Investigación y Desarrollo Agroalimentario. Centro de Biotecnología Animal)
Prieto, Miguel (SERIDA. Servicio Regional de Investigación y Desarrollo Agroalimentario. Centro de Biotecnología Animal)
Pérez De Val, Bernat (Universitat Autònoma de Barcelona. Centre de Recerca en Sanitat Animal)

Data: 2017
Resum: Animal tuberculosis (TB) is a complex animal health problem that causes disruption to trade and significant economic losses. TB involves a multi-host system where sheep, traditionally considered a rare host of this infection, have been recently included. The aims of this study were to develop an experimental TB infection model in sheep with a Mycobacterium caprae field strain isolated from a tuberculous diseased ewe, and to use this to evaluate the safety and efficacy of two vaccines against TB in sheep, the live-attenuated M. bovis BCG vaccine (Danish strain) and a heat-inactivated M. bovis (HIMB) vaccine. Methods: Eighteen 2 month-old lambs were experimentally challenged with M. caprae by the endotracheal route (1. 5 × 10 CFU). They were separated per treatment group into parenterally vaccinated with a live BCG Danish strain vaccine (n = 6), orally vaccinated with a suspension of HIMB (n = 6) and unvaccinated controls (n = 6). Clinical, immunological, pathological and bacteriological parameters of infection were measured. Results: All lambs were successfully infected and developed gross TB lesions in the respiratory system. The BCG vaccine conferred considerable protection against experimental TB in lambs, as measured by a reduction of the gross lesion volumes and bacterial load. However, HIMB vaccinated animals did not show protection. Conclusions: This study proposes a reliable new experimental model for a better understanding of tuberculosis in sheep. BCG vaccination offers an effective prospect for controlling the disease. Moreover alternative doses and/or routes of administration should be considered to evaluate the efficacy of the HIMB vaccine candidate.
Nota: Número d'acord de subvenció INIA/RTA2014-0002-C02-01
Drets: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Llengua: Anglès.
Document: article ; recerca ; publishedVersion
Matèria: Hydrogen-Ion Concentration
Publicat a: PLoS ONE, Vol. 12 Núm. 7 (july 2017) , p. e0180546, ISSN 1932-6203

DOI: 10.1371/journal.pone.0180546
PMID: 28678885


16 p, 3.9 MB

El registre apareix a les col·leccions:
Documents de recerca > Documents dels grups de recerca de la UAB > Centres i grups de recerca (producció científica) > Ciències de la salut i biociències > Centre de Recerca en Sanitat Animal (CReSA-IRTA)
Articles > Articles de recerca
Articles > Articles publicats

 Registre creat el 2019-01-21, darrera modificació el 2019-02-22



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