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Biomphalaria glabrata metallothionein : Lacking metal specificity of the protein and missing gene upregulation suggest metal sequestration by exchange instead of through selective binding
Niederwanger, Michael (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences Innsbruck)
Calatayud, Sara (Universitat de Barcelona. Departament de Biologia evolutiva. Institut de Recerca de la Biodiversitat)
Zerbe, Oliver (University of Zürich. Department of Chemistry)
Atrian, Silvia (Universitat de Barcelona. Departament de Biologia evolutiva. Institut de Recerca de la Biodiversitat)
Albalat, Ricard (Universitat de Barcelona. Departament de Biologia evolutiva. Institut de Recerca de la Biodiversitat)
Capdevila Vidal, Mercè (Universitat Autònoma de Barcelona. Departament de Química)
Palacios Bonilla, Òscar (Universitat Autònoma de Barcelona. Departament de Química)
Dallinger, Reinherd (University of Innsbruck. Institute of Zoology and Center of Molecular Biosciences Innsbruck)

Date: 2017
Abstract: The wild-type metallothionein (MT) of the freshwater snail Biomphalaria glabrata and a natural allelic mutant of it in which a lysine residue was replaced by an asparagine residue, were recombinantly expressed and analyzed for their metal-binding features with respect to Cd, Zn and Cu, applying spectroscopic and mass-spectrometric methods. In addition, the upregulation of the Biomphalaria glabrata MT gene was assessed by quantitative real-time detection PCR. The two recombinant proteins revealed to be very similar in most of their metal binding features. They lacked a clear metal-binding preference for any of the three metal ions assayed—which, to this degree, is clearly unprecedented in the world of Gastropoda MTs. There were, however, slight differences in copper-binding abilities between the two allelic variants. Overall, the missing metal specificity of the two recombinant MTs goes hand in hand with lacking upregulation of the respective MT gene. This suggests that in vivo, the Biomphalaria glabrata MT may be more important for metal replacement reactions through a constitutively abundant form, rather than for metal sequestration by high binding specificity. There are indications that the MT of Biomphalaria glabrata may share its unspecific features with MTs from other freshwater snails of the Hygrophila family.
Note: Número d'acord de subvenció AGAUR/2014/SGR-423
Note: Número d'acord de subvenció MINECO/BIO2015-67358-C2-1-P
Note: Número d'acord de subvenció MINECO/BIO2015-67358-C2-2-P
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Metallothionein ; Metal-binding ; Zinc ; Copper ; Cadmium ; Biomphalaria glabrata ; Gastropoda ; Hygrophila
Published in: International Journal of Molecular Sciences, Vol. 18, Num. 7 (July 2017) , art. 1457, ISSN 1422-0067

DOI: 10.3390/ijms18071457
PMID: 28684706


15 p, 2.3 MB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2019-01-31, last modified 2019-03-29



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