Biochemistry of malaria parasite infected red blood cells by X-ray microscopy
Kapishnikov, Sergey (University of Copenhagen)
Yang, Y. (European Synchrotron Radiation Facility)
Cloetens, Peter (European Synchrotron Radiation Facility)
Pereiro, Eva (ALBA Laboratori de Llum de Sincrotró)
Awamu Ndonglack, Florence (Humboldt University)
Matuschewski, Kai (Humboldt University)
Als-Nielsen, Jens (University of Copenhagen)
Leiserowitz, Leslie (Weizmann Institute of Science (Israel))

Date:	2017
Abstract:	Red blood cells infected by the malaria parasite Plasmodium falciparum are correlatively imaged by tomography using soft X-rays as well as by scanning hard nano-X-ray beam to obtain fluorescence maps of various elements such as S and Fe. In this way one can deduce the amount of Fe bound either in hemoglobin or in hemozoin crystals in the digestive vacuole of the malaria parasite as well as determine the hemoglobin concentrations in the cytosols of the red blood cell and of the parasite. Fluorescence map of K shows that in the parasite's schizont stage the K concentration in the red blood cell cytosol is diminished by a factor of seven relative to a pristine red blood cell but the total amount of K in the infected red blood cell is the same as in the pristine red blood cell.
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Electron Probe Microanalysis ; Erythrocytes ; Hemeproteins ; Hemoglobins ; Humans ; Iron ; Malaria, Falciparum ; Plasmodium falciparum ; Potassium ; Sulfur
Published in:	Scientific reports, Vol. 7 (April 2017) , art. 802, ISSN 2045-2322

DOI: 10.1038/s41598-017-00921-2
PMID: 28400621

9 p, 2.7 MB

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