Photoluminescent lateral flow based on non-radiative energy transfer for protein detection in human serum
Zamora Gálvez, Alejandro (Institut Català de Nanociència i Nanotecnologia)
Morales-Narváez, Eden (Institut Català de Nanociència i Nanotecnologia)
Romero, Javier (Institut Català de Nanociència i Nanotecnologia)
Merkoçi, Arben (Institut Català de Nanociència i Nanotecnologia)

Data:	2018
Resum:	A new paper-based lateral flow immunoassay configuration was engineered and investigated. The assay is intended for the detection of a model protein in human serum, that is, human immunoglobulin G, with the aim to demonstrate a virtually universal protein detection platform. Once the sample is added in the strip, the analyte is selectively captured by antibody-decorated silica beads (Ab-SiO) onto the conjugate pad and the sample flows by capillarity throughout the strip until reaching the test line, where a sandwich-like immunocomplex takes place due to the presence of antibody-functionalized QDs (Ab-QDs) onto the test line. Eventually, GO is added as a revealing agent and the photoluminescence of those sites protected by the complex Ab-SiO/Antigen/Ab-QDs will not be quenched, whereas those photoluminescent sites directly exposed are expected to be quenched by GO, including the control line, made of bare QDs, reporting that the assay occurred successfully. Hence, the photoluminescence of the test line is modulated by the formation of sandwich-like immunocomplexes. The proposed device achieves a limit of detection (LOD) of 1. 35 ng mL in standard buffer, which is lower when compared with conventional lateral flow technology reported by gold nanoparticles, including other amplification strategies. Moreover, the resulting device was proven useful in human serum analysis, achieving a LOD of 6. 30 ng mL in this complex matrix. This low-cost disposable and easy-to-use device will prove valuable for portable and automated diagnostics applications, and can be easily transferred to other analytes such as clinically relevant protein biomarkers.
Ajuts:	Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-260 Ministerio de Economía y Competitividad SEV-2013-0295
Drets:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Llengua:	Anglès
Document:	Article ; recerca ; Versió acceptada per publicar
Matèria:	Fluorescence ; Graphene oxide ; Lateral flow immunoassay ; Protein detection ; Quantum dots
Publicat a:	Biosensors & bioelectronics, Vol. 100 (February 2018) , p. 208-213, ISSN 1873-4235

DOI: 10.1016/j.bios.2017.09.013

Postprint 20 p, 1.4 MB

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