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Microencapsulation with alginate/CaCO 3 : A strategy for improved phage therapy
Colom, Joan (Colom Farran) (Universitat Autònoma de Barcelona. Departament de Genètica i Microbiologia)
Cano Sarabia, Antonia María (Institut Català de Nanociència i Nanotecnologia)
Otero Carrera, Jennifer (Universitat Autònoma de Barcelona. Departament de Genètica i Microbiologia)
Aríñez Soriano, Javier (Institut Català de Nanociència i Nanotecnologia)
Cortés Garmendia, M. Pilar (Universitat Autònoma de Barcelona. Departament de Genètica i Microbiologia)
Maspoch Comamala, Daniel (Institut Català de Nanociència i Nanotecnologia)
Llagostera Casas, Montserrat (Universitat Autònoma de Barcelona. Departament de Genètica i Microbiologia)

Date: 2017
Abstract: Bacteriophages are promising therapeutic agents that can be applied to different stages of the commercial food chain. In this sense, bacteriophages can be orally administered to farm animals to protect them against intestinal pathogens. However, the low pH of the stomach, the activities of bile and intestinal tract enzymes limit the efficacy of the phages. This study demonstrates the utility of an alginate/CaCO encapsulation method suitable for bacteriophages with different morphologies and to yield encapsulation efficacies of ∼100%. For the first time, a cocktail of three alginate/CaCO-encapsulated bacteriophages was administered as oral therapy to commercial broilers infected with Salmonella under farm-like conditions. Encapsulation protects the bacteriophages against their destruction by the gastric juice. Phage release from capsules incubated in simulated intestinal fluid was also demonstrated, whereas encapsulation ensured sufficient intestinal retention of the phages. Moreover, the small size of the capsules (125-150 μm) enables their use in oral therapy and other applications in phage therapy. This study evidenced that a cocktail of the three alginate/CaCO-encapsulated bacteriophages had a greater and more durable efficacy than a cocktail of the corresponding non-encapsulated phages in as therapy in broilers against Salmonella, one of the most common foodborne pathogen.
Note: Altres ajuts: La Caixa and the Associació Catalana d'Universitats Públiques 2010ACUP00300
Note: Número d'acord de subvenció AGAUR/2014/SGR-572
Note: Número d'acord de subvenció AGAUR/2010/VALOR00114
Note: Número d'acord de subvenció MINECO/BIO2016-77011-R
Note: Número d'acord de subvenció MINECO/SEV-2013-0295
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès.
Document: article ; recerca ; publishedVersion
Subject: Alginates ; Animals ; Body Fluids ; Calcium Carbonate ; Cecum ; Chickens ; Drug Compounding ; Glucuronic Acid ; Hexuronic Acids ; Imaging, Three-Dimensional ; Phage Therapy ; Salmonella ; Salmonella Infections, Animal
Published in: Scientific reports, Vol. 7 (January 2017) , art. 41441, ISSN 2045-2322

DOI: 10.1038/srep41441
PMID: 28120922

10 p, 1004.6 KB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles

 Record created 2019-04-01, last modified 2019-11-06

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