Web of Science: 12 citas, Scopus: 15 citas, Google Scholar: citas,
Bacterial endotoxin (lipopolysaccharide) binds to the surface of gold nanoparticles, interferes with biocorona formation and induces human monocyte inflammatory activation
Li, Yang (Hebei Normal University)
Shi, Zhenzhen (Kansas State University. Department of Anatomy and Physiology)
Radauer-Preiml, Isabella (University of Salzburg. Department of Molecular Biology)
Andosch, Ancuela (University of Salzburg. Department of Molecular Biology)
Casals, Eudald (Vall d'Hebron Institut de Recerca)
Luetz-Meindl, Ursula (University of Salzburg. Department of Molecular Biology)
Cobaleda-Siles, Macarena (Vall d'Hebron Institut de Recerca)
Lin, Zhoumeng (Kansas State University. Department of Anatomy and Physiology)
Jaberi-Douraki, Majid (Kansas State University. Department of Anatomy and Physiology)
Italiani, Paola (National Research Council)
Horejs-Hoeck, Jutta (University of Salzburg. Department of Molecular Biology)
Himly, Martin (University of Salzburg. Department of Molecular Biology)
Monteiro-Riviere, Nancy A. (Kansas State University. Department of Anatomy and Physiology)
Duschl, Albert (University of Salzburg. Department of Molecular Biology)
Puntes, Víctor (Institut Català de Nanociència i Nanotecnologia)
Boraschi, Diana (National Research Council)

Fecha: 2017
Resumen: Nanoparticles (NPs) are easily contaminated by bacterial endotoxin (lipopolysaccharide [LPS]). The presence of LPS can be responsible for many immune/inflammatory effects attributed to NPs. In this study, we examined the effects of LPS adsorption on the NP surface on the formation of a biocorona in biological fluids and on the subsequent inflammation-inducing activity of NPs. Different gold (Au) NPs with sizes ranging from 10 to 80 nm and with different surface functionalization (sodium citrate, lipoic acid, and branched polyethyleneimine (BPEI), or polyethylene glycol (PEG)) were exposed to E. coli LPS under different conditions. The binding capacity of LPS to the surface of AuNPs was dose- and time-dependent. LPS attached to sodium citrate and lipoic acid coatings, but did not adhere to BPEI- or PEG-coated NPs. By computational simulation, the binding of LPS to AuNPs seems to follow the Langmuir absorption isotherm. The presence of LPS on AuNP surface interfered and caused a decrease in the formation of the expected biomolecular corona upon incubation in human plasma. LPS-coated AuNPs, but not the LPS-free NPs, induced significant inflammatory responses in vitro. Notably, while free LPS did also induce an anti-inflammatory response, LPS bound to NPs appeared unable to do so. In conclusion, the unintentional adsorption of LPS onto the NP surface can affect the biocorona formation and the inflammatory properties of NPs. Thus, for an accurate interpretation of NP interactions with cells, it is extremely important to be able to distinguish the intrinsic NP biological effects from those caused by biologically active contaminants such as endotoxin.
Nota: Número d'acord de subvenció EC/FP7/264506
Nota: Número d'acord de subvenció EC/FP7/263147
Nota: Número d'acord de subvenció EC/H2020/671881
Derechos: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Lengua: Anglès.
Documento: article ; recerca ; publishedVersion
Materia: Nano-immunosafety ; Endotoxin contamination ; Biocorona modulation ; Gold nanoparticles ; Inflammatory effects
Publicado en: Nanotoxicology, Vol. 11, Issue 9-10 (December 2017) , p. 1157-1175, ISSN 1743-5404

DOI: 10.1080/17435390.2017.1401142


20 p, 3.7 MB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
Artículos > Artículos de investigación
Artículos > Artículos publicados

 Registro creado el 2019-06-03, última modificación el 2019-12-18



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