Gadolinium-functionalised multi-walled carbon nanotubes as a T1 contrast agent for MRI cell labelling and tracking
Servant, Ania (University of Manchester)
Jacobs, Igor (Eindhoven University of Technology)
Bussy, Cyrill (University of Manchester)
Fabbro, Chiara (University of Trieste. Department of Pharmaceutical Sciences)
Da Ros, Tatiana (University of Trieste. Department of Pharmaceutical Sciences)
Pach, Elzbieta (Institut Català de Nanociència i Nanotecnologia)
Ballesteros, Belén (Institut Català de Nanociència i Nanotecnologia)
Prato, Maurizio (University of Trieste. Department of Pharmaceutical Sciences)
Nicolay, Klaas (Eindhoven University of Technology. Department of Biomedical Engineering)
Kostarelos, Kostas (University of Manchester)

Date:	2016
Abstract:	The development of efficient contrast agents for cell labelling coupled with powerful medical imaging techniques is of great interest for monitoring cell trafficking with potential clinical applications such as organ repair and regenerative medicine. In this paper, functionalised multi-walled carbon nanotubes (MWNTs) were engineered for cell labelling in T-weighted MRI applications. These sophisticated constructs were covalently functionalised with the gadolinium (Gd) chelating agent, diethylene triamine pentaacetic acid (DTPA), enabling tight attachment of Gd atoms onto the nanotube surface. The resulting Gd-labelled MWNTs were found to be stable over 2 weeks in water and mouse serum and high payload of Gd atoms could be loaded onto the nanotubes. The r relaxivity of the Gd-MWNTs was a 3-fold higher than of the clinically approved T contrast agent Magnevist at a magnetic field strength of 7T. The contrast efficiency, expressed as the r relaxivity, of the Gd-MWNTs in Human Umbilical Vein Endothelial cells (HUVEC) was investigated at 7T and was found to be around 6. 6 mM s. There was no reduction of the contrast efficiency after internalisation in HUVECs, which was imparted to the ability of carbon nanotubes to translocate the cell membrane.
Grants:	European Commission 276051
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	MWNTs ; Gadolinium ; HUVEC ; Cell labelling ; T1-weighted MRI ; Endosomal entrapment
Published in:	Carbon, Vol. 97 (February 2016) , p. 126-133, ISSN 0008-6223

DOI: 10.1016/j.carbon.2015.08.051

8 p, 1.5 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles