Label-Free and Real-Time Detection of Tuberculosis in Human Urine Samples Using a Nanophotonic Point-of-Care Platform
Ramirez Priego, Patricia (Institut Català de Nanociència i Nanotecnologia)
Martens, Daan (Ghent University)
Elamin, Ayssar A. (LIONEX Diagnostics and Therapeutics GmbH)
Soetaert, Pieterjan (Trinean NV)
Van Roy, Wim (Imec)
Vos, Rita (Imec)
Anton, Birgit (Microfluidic ChipShop GmbH)
Bockstaele, Ronny (Trinean NV)
Becker, Holger (Microfluidic ChipShop GmbH)
Singh, Mahavir (LIONEX Diagnostics and Therapeutics GmbH)
Bienstman, Peter (Ghent University)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Date:	2018
Abstract:	Tuberculosis (TB) is the leading global cause of death from a single infectious agent. Registered incidence rates are low, especially in low-resource countries with weak health systems, due to the disadvantages of current diagnostic techniques. A major effort is directed to develop a point-of-care (POC) platform to reduce TB deaths with a prompt and reliable low-cost technique. In the frame of the European POCKET Project, a novel POC platform for the direct and noninvasive detection of TB in human urine was developed. The photonic sensor chip is integrated in a disposable cartridge and is based on a highly sensitive Mach-Zehnder Interferometer (MZI) transducer combined with an on-chip spectral filter. The required elements for the readout are integrated in an instrument prototype, which allows real-time monitoring and data processing. In this work, the novel POC platform has been employed for the direct detection of lipoarabinomannan (LAM), a lipopolysaccharide found in the mycobacterium cell wall. After the optimization of several parameters, a limit of detection of 475 pg/mL (27. 14 pM) was achieved using a direct immunoassay in undiluted human urine in less than 15 min. A final validation of the technique was performed using 20 clinical samples from TB patients and healthy donors, allowing the detection of TB in people regardless of HIV coinfection. The results show excellent correlation to those obtained with standard techniques. These promising results demonstrate the high sensitivity, specificity and applicability of our novel POC platform, which could be used during routine check-ups in developing countries.
Grants:	European Commission 610389 Ministerio de Economía y Competitividad SEV-2013-0295
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Subject:	Point-of-care ; Nanophotonic biosensor ; LAM ; Biomarker ; Urine ; Diagnosis ; Tuberculosis
Published in:	ACS Sensors, Vol. 3, Núm. 10 (September 2018) , p. 2079-2086, ISSN 2379-3694

DOI: 10.1021/acssensors.8b00393
PMID: 30269480

Preprint 10 p, 1.1 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles