A low-cost integrated biosensing platform based on SiN nanophotonics for biomarker detection in urine
Martens, Daan (Ghent University)
Ramírez Priego, Patricia (Institut Català de Nanociència i Nanotecnologia)
Murib, M.S. (Ghent University)
Elamin, Ayssar A. (LIONEX Diagnostics and Therapeutics GmbH)
González-Guerrero, Ana Belén (Institut Català de Nanociència i Nanotecnologia)
Stehr, M. (LIONEX Diagnostics and Therapeutics GmbH)
Jonas, F. (LIONEX Diagnostics and Therapeutics GmbH)
Anton, Birgit (Microfluidic ChipShop GmbH)
Hlawatsch, N. (Microfluidic ChipShop GmbH)
Soetaert, Pieterjan (Trinean NV)
Vos, Rita (Imec)
Stassen, A. (Imec)
Severi, S. (Imec)
Van Roy, Wim (Imec)
Bockstaele, Ronny (Trinean NV)
Becker, Holger (Microfluidic ChipShop GmbH)
Singh, M. (LIONEX Diagnostics and Therapeutics GmbH)
Lechuga, Laura (Institut Català de Nanociència i Nanotecnologia)
Bienstman, Peter (Ghent University)

Fecha:	2018
Resumen:	We present a low-cost integrated nanophotonic lab-on-a-chip platform suitable for point-of-care (POC) biomarker analysis. The sensor chip included in the platform contains multiplexed Mach-Zehnder interferometers with an on-chip optical spectral analyser consisting of an arrayed-waveguide grating. The sensor chip is fabricated in silicon nitride material, which makes it compatible with consumer-electronics-grade sources and detectors, leading to the possibility of low-cost instrumentation. The nanophotonic sensor chip exhibits a detection limit of 6 × 10 RIU (Refractive Index Units), which is in the same order of magnitude as the reported values for state-of-the-art evanescent wave sensors. The sensor chip is biofunctionalised with specific bioreceptors and integrated into a polymer microfluidic cartridge. The POC instrumentation platform contains optical excitation and read-out sub-systems and dedicated on-board software for real-time analysis of patient samples. To demonstrate the versatility of the platform, we present results both on the detection of an antigen related to tuberculosis directly in urine samples using a laboratory prototype and on the detection of a protein biomarker (CRP) related to inflammation using the integrated instrument.
Nota:	Número d'acord de subvenció EC/FP7/610389
Nota:	Número d'acord de subvenció AGAUR/2014/SGR-624
Nota:	Número d'acord de subvenció MINECO/SEV-2013-0295
Derechos:	Tots els drets reservats.
Lengua:	Anglès
Documento:	article ; recerca ; submittedVersion ; acceptedVersion
Materia:	Biosensing platforms ; Evanescent wave sensors ; Nitride materials ; On board softwares ; Polymer microfluidics ; Real time analysis ; Refractive index units ; State of the art
Publicado en:	Analytical methods, Vol. 10, Issue 25 (July 2018) , p. 3066-3073, ISSN 1759-9679

DOI: 10.1039/c8ay00666k

Preprint 9 p, 1.1 MB

Postprint 17 p, 991.7 KB

El registro aparece en las colecciones:
Documentos de investigación > Documentos de los grupos de investigación de la UAB > Centros y grupos de investigación (producción científica) > Ciencias > Institut Català de Nanociència i Nanotecnologia (ICN2)
Artículos > Artículos de investigación
Artículos > Artículos publicados