Delegating sex : differential gene expression in stolonizing syllids uncovers the hormonal control of reproduction
Álvarez-Campos, Patricia (Universidad Autónoma de Madrid. Departamento de Biología (Zoología))
Kenny, Nathan J. (Natural History Museum (Londres, Regne Unit). Department of Life Sciences)
Verdes, Aida (Universidad Autónoma de Madrid. Departamento de Biología (Zoología))
Fernández García, Rosa (Centre de Recerca en Agrigenòmica)
Novo, Marta (Universidad Complutense de Madrid. Departamento de Biodiversidad, Ecología y Evolución)
Giribet, Gonzalo (Harvard University. Museum of Comparative Zoology)
Riesgo, Ana (City University of New York. The Graduate Center. Department of Biology)

Date:	2019
Abstract:	Stolonization in syllid annelids is a unique mode of reproduction among animals. During the breeding season, a structure resembling the adult but containing only gametes, called stolon, is formed generally at the posterior end of the animal. When stolons mature, they detach from the adult and gametes are released into the water column. The process is synchronized within each species, and it has been reported to be under environmental and endogenous control, probably via endocrine regulation. To further understand reproduction in syllids and to elucidate the molecular toolkit underlying stolonization, we generated Illumina RNA-seq data from different tissues of reproductive and nonreproductive individuals of Syllis magdalena and characterized gene expression during the stolonization process. Several genes involved in gametogenesis (ovochymase, vitellogenin, testis-specific serine/threonine-kinase), immune response (complement receptor 2), neuronal development (tyrosine-protein kinase Src42A), cell proliferation (alpha-1D adrenergic receptor), and steroid metabolism (hydroxysteroid dehydrogenase 2) were found differentially expressed in the different tissues and conditions analyzed. In addition, our findings suggest that several neurohormones, such as methyl farnesoate, dopamine, and serotonin, might trigger stolon formation, the correct maturation of gametes and the detachment of stolons when gametogenesis ends. The process seems to be under circadian control, as indicated by the expression patterns of r-opsins. Overall, our results shed light into the genes that orchestrate the onset of gamete formation and improve our understanding of how some hormones, previously reported to be involved in reproduction and metamorphosis processes in other invertebrates, seem to also regulate reproduction via stolonization.
Grants:	European Commission 227799
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Subject:	Transcriptomics ; Stolonizing syllids ; Reproduction ; Hormonal control
Published in:	Genome biology and evolution, Vol. 11, issue 1 (Jan. 2019) , p. 295-318, ISSN 1759-6653

DOI: 10.1093/gbe/evy265
PMID: 30535381

24 p, 2.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles