An automated optofluidic biosensor platform combining interferometric sensors and injection moulded microfluidics
Szydzik, C. (RMIT University. School of Engineering)
Fernandez-Gavela, Adrian (Institut Català de Nanociència i Nanotecnologia)
Herranz, Sonia (Institut Català de Nanociència i Nanotecnologia)
Roccisano, J. (RMIT University. School of Engineering)
Knoerzer, M. (RMIT University. School of Engineering)
Thurgood, P. (RMIT University. School of Engineering)
Khoshmanesh, K. (RMIT University. School of Engineering)
Mitchell, Arnan (RMIT University. School of Engineering)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Date:	2017
Abstract:	A primary limitation preventing practical implementation of photonic biosensors within point-of-care platforms is their integration with fluidic automation subsystems. For most diagnostic applications, photonic biosensors require complex fluid handling protocols; this is especially prominent in the case of competitive immunoassays, commonly used for detection of low-concentration, low-molecular weight biomarkers. For this reason, complex automated microfluidic systems are needed to realise the full point-of-care potential of photonic biosensors. To fulfil this requirement, we propose an on-chip valve-based microfluidic automation module, capable of automating such complex fluid handling. This module is realised through application of a PDMS injection moulding fabrication technique, recently described in our previous work, which enables practical fabrication of normally closed pneumatically actuated elastomeric valves. In this work, these valves are configured to achieve multiplexed reagent addressing for an on-chip diaphragm pump, providing the sample and reagent processing capabilities required for automation of cyclic competitive immunoassays. Application of this technique simplifies fabrication and introduces the potential for mass production, bringing point-of-care integration of complex automated microfluidics into the realm of practicality. This module is integrated with a highly sensitive, label-free bimodal waveguide photonic biosensor, and is demonstrated in the context of a proof-of-concept biosensing assay, detecting the low-molecular weight antibiotic tetracycline.
Grants:	European Commission 614010 Agència de Gestió d'Ajuts Universitaris i de Recerca 2014/SGR-624 Ministerio de Economía y Competitividad SEV-2013-0295
Rights:	Tots els drets reservats.
Language:	Anglès
Document:	Article ; recerca ; Versió sotmesa a revisió
Subject:	Biosensing Techniques ; Immunoassay ; Lab-On-A-Chip Devices ; Microfluidic Analytical Techniques ; Optics and Photonics ; Point-of-Care Systems
Published in:	Lab on a chip, Vol. 17, Issue 16 (August 2017) , p. 2793-2804, ISSN 1473-0189

DOI: 10.1039/c7lc00524e

Preprint 20 p, 10.4 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles