Web of Science: 34 citations, Scopus: 37 citations, Google Scholar: citations,
Highly sensitive dendrimer-based nanoplasmonic biosensor for drug allergy diagnosis
Soler Aznar, Maria (Institut Català de Nanociència i Nanotecnologia)
Mesa Antúnez, Pablo (Universidad de Málaga)
Estévez Alberola, Maria Carmen (Institut Català de Nanociència i Nanotecnologia)
Ruiz Sánchez, Antonio Jesús (Universidad de Málaga)
Otte Ortiz, Marinus Albertus (Institut Català de Nanociència i Nanotecnologia)
Sepúlveda Martínez, Borja (Institut Català de Nanociència i Nanotecnologia)
Collado, Daniel (Universidad de Málaga)
Mayorga, Cristobalina (Andalusian Centre for Nanomedicine and Biotechnology-BIONAND)
Torres, María José (Andalusian Centre for Nanomedicine and Biotechnology-BIONAND)
Pérez Inestrosa, Ezequiel (Universidad de Málaga)
Lechuga, Laura M. (Institut Català de Nanociència i Nanotecnologia)

Date: 2015
Abstract: A label-free biosensing strategy for amoxicillin (AX) allergy diagnosis based on the combination of novel dendrimer-based conjugates and a recently developed nanoplasmonic sensor technology is reported. Gold nanodisks were functionalized with a custom-designed thiol-ending-polyamido-based dendron (d-BAPAD) peripherally decorated with amoxicilloyl (AXO) groups (d-BAPAD-AXO) in order to detect specific IgE generated in patient's serum against this antibiotic during an allergy outbreak. This innovative strategy, which follows a simple one-step immobilization procedure, shows exceptional results in terms of sensitivity and robustness, leading to a highly-reproducible and long-term stable surface which allows achieving extremely low limits of detection. Moreover, the viability of this biosensor approach to analyze human biological samples has been demonstrated by directly analyzing and quantifying specific anti-AX antibodies in patient's serum without any sample pretreatment. An excellent limit of detection (LoD) of 0. 6. ng/mL (i. e. 0. 25. kU/L) has been achieved in the evaluation of clinical samples evidencing the potential of our nanoplasmonic biosensor as an advanced diagnostic tool to quickly identify allergic patients. The results have been compared and validated with a conventional clinical immunofluorescence assay (ImmunoCAP test), confirming an excellent correlation between both techniques. The combination of a novel compact nanoplasmonic platform and a dendrimer-based strategy provides a highly sensitive label free biosensor approach with over two times better detectability than conventional SPR. Both the biosensor device and the carrier structure hold great potential in clinical diagnosis for biomarker analysis in whole serum samples and other human biological samples.
Note: Número d'acord de subvenció MICINN/CTQ2010-20303
Note: Número d'acord de subvenció MINECO/TEC2099-08729
Note: Número d'acord de subvenció MINECO/SEV2013-0295
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades. Creative Commons
Language: Anglès.
Document: article ; recerca ; acceptedVersion
Subject: Nanoplasmonic biosensor ; Gold nanodisks ; Dendrimer ; Allergy diagnosis ; IgE quantification ; Serum clinical sample ; Label-free detection
Published in: Biosensors and bioelectronics, Vol. 66 (April 2015) , p. 115-123, ISSN 1873-4235

DOI: 10.1016/j.bios.2014.10.081


Postprint
29 p, 1.3 MB

The record appears in these collections:
Research literature > UAB research groups literature > Research Centres and Groups (scientific output) > Experimental sciences > Catalan Institute of Nanoscience and Nanotechnology (ICN2)
Articles > Research articles
Articles > Published articles

 Record created 2019-09-23, last modified 2019-10-25



   Favorit i Compartir