Highly sensitive dendrimer-based nanoplasmonic biosensor for drug allergy diagnosis
Soler Aznar, Maria (Institut Català de Nanociència i Nanotecnologia)
Mesa Antúnez, Pablo (Universidad de Málaga)
Estévez, M. Carmen (Institut Català de Nanociència i Nanotecnologia)
Ruiz Sánchez, Antonio Jesús (Universidad de Málaga)
Otte Ortiz, Marinus Albertus (Institut Català de Nanociència i Nanotecnologia)
Sepúlveda, Borja (Institut Català de Nanociència i Nanotecnologia)
Collado, Daniel (Universidad de Málaga)
Mayorga, Cristobalina (Centro Andaluz de Nanomedicina y Biotecnología (BIONAND))
Torres, María José (Centro Andaluz de Nanomedicina y Biotecnología (BIONAND))
Pérez Inestrosa, Ezequiel (Universidad de Málaga)
Lechuga, Laura M (Institut Català de Nanociència i Nanotecnologia)

Fecha:	2015
Resumen:	A label-free biosensing strategy for amoxicillin (AX) allergy diagnosis based on the combination of novel dendrimer-based conjugates and a recently developed nanoplasmonic sensor technology is reported. Gold nanodisks were functionalized with a custom-designed thiol-ending-polyamido-based dendron (d-BAPAD) peripherally decorated with amoxicilloyl (AXO) groups (d-BAPAD-AXO) in order to detect specific IgE generated in patient's serum against this antibiotic during an allergy outbreak. This innovative strategy, which follows a simple one-step immobilization procedure, shows exceptional results in terms of sensitivity and robustness, leading to a highly-reproducible and long-term stable surface which allows achieving extremely low limits of detection. Moreover, the viability of this biosensor approach to analyze human biological samples has been demonstrated by directly analyzing and quantifying specific anti-AX antibodies in patient's serum without any sample pretreatment. An excellent limit of detection (LoD) of 0. 6. ng/mL (i. e. 0. 25. kU/L) has been achieved in the evaluation of clinical samples evidencing the potential of our nanoplasmonic biosensor as an advanced diagnostic tool to quickly identify allergic patients. The results have been compared and validated with a conventional clinical immunofluorescence assay (ImmunoCAP test), confirming an excellent correlation between both techniques. The combination of a novel compact nanoplasmonic platform and a dendrimer-based strategy provides a highly sensitive label free biosensor approach with over two times better detectability than conventional SPR. Both the biosensor device and the carrier structure hold great potential in clinical diagnosis for biomarker analysis in whole serum samples and other human biological samples.
Ayudas:	Ministerio de Ciencia e Innovación CTQ2010-20303 Ministerio de Economía y Competitividad TEC2099-08729 Ministerio de Economía y Competitividad SEV2013-0295
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió acceptada per publicar
Materia:	Nanoplasmonic biosensor ; Gold nanodisks ; Dendrimer ; Allergy diagnosis ; IgE quantification ; Serum clinical sample ; Label-free detection
Publicado en:	Biosensors & bioelectronics, Vol. 66 (April 2015) , p. 115-123, ISSN 1873-4235

DOI: 10.1016/j.bios.2014.10.081

Postprint 29 p, 1.3 MB

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