The proteins encoded by the pogo-like Lemi1 element bind the TIRs and subterminal repeated motifs of the Arabidopsis Emigrant MITE : Consequences for the transposition mechanism of MITEs
Loot, Céline (Institut de Recerca i Tecnologia Agroalimentàries)
Santiago, Néstor (Institut de Recerca i Tecnologia Agroalimentàries)
Sanz, Alicia (Institut de Recerca i Tecnologia Agroalimentàries)
Casacuberta i Suñer, Josep M. 1962- (Institut de Recerca i Tecnologia Agroalimentàries)

Fecha:	2006
Resumen:	MITEs (miniature inverted-repeated transposable elements) are a particular class of defective DNA transposons usually present within genomes as high copy number populations of highly homogeneous elements. Although an active MITE, the mPing element, has recently been characterized in rice, the transposition mechanism of MITEs remains unknown. It has been proposed that transposases of related transposons could mobilize MITEs in trans. Moreover, it has also been proposed that the presence of conserved terminal inverted-repeated (TIR) sequences could be the only requirement of MITEs for mobilization, allowing divergent or unrelated elements to be mobilized by a particular transposase. We present here evidence for a recent mobility of the Arabidopsis Emigrant MITE and we report on the capacity of the proteins encoded by the related Lemi1 transposon, a pogo-related element, to specifically bind Emigrant elements. This suggests that Lemi 1 could mobilize Emigrant elements and makes the Lemi1/Emigrant couple an ideal system to study the transposition mechanism of MITEs. Our results show that Lemi1 proteins bind Emigrant TIRs but also bind cooperatively to subterminal repeated motifs. The requirement of internal sequences for the formation of proper DNA/protein structure could affect the capacity of divergent MITEs to be mobilized by distantly related transposases.
Ayudas:	Ministerio de Ciencia y Tecnología BIO2003-01211
Derechos:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original.
Lengua:	Anglès
Documento:	Article ; recerca ; Versió publicada
Publicado en:	Nucleic acids research, Vol. 34, Issue 18 (October 2006) , p. 5238-5246, ISSN 1362-4962

DOI: 10.1093/nar/gkl688
PMID: 17003053

9 p, 1.4 MB

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