Genomic characterization of putative allergen genes in peach/almond and their synteny with apple
Chen, Lin (Zhejiang University. Allergy Research Center)
Zhang, Shuiming (Anhui Agricultural University. Key Laboratory of Pomology)
Illa, Eudald (Centre de Recerca en Agrigenòmica)
Song, Lijuan (Zhejiang University. Institute of Fruit Science)
Wu, Shandong (Zhejiang University. Allergy Research Center)
Howad, Werner (Centre de Recerca en Agrigenòmica)
Arús i Gorina, Pere (Centre de Recerca en Agrigenòmica)
Van de Weg, Eric (Wageningen University and Research Centre. Plant Research International)
Chen, Kunsong (Zhejiang University. Institute of Fruit Science)
Gao, Zhongshan (Zhejiang University. Allergy Research Center)

Date:	2008
Abstract:	Background: Fruits from several species of the Rosaceae family are reported to cause allergic reactions in certain populations. The allergens identified belong to mainly four protein families: pathogenesis related 10 proteins, thaumatin-like proteins, lipid transfer proteins and profilins. These families of putative allergen genes in apple (Mal d 1 to 4) have been mapped on linkage maps and subsequent genetic study on allelic diversity and hypoallergenic traits has been carried out recently. In peach (Prunus persica), these allergen gene families are denoted as Pru p 1 to 4 and for almond (Prunus dulcis)Pru du 1 to 4. Genetic analysis using current molecular tools may be helpful to establish the cause of allergenicity differences observed among different peach cultivars. This study was to characterize putative peach allergen genes for their genomic sequences and linkage map positions, and to compare them with previously characterized homologous genes in apple (Malus domestica). Results: Eight Pru p/du 1 genes were identified, four of which were new. All the Pru p/du 1 genes were mapped in a single bin on the top of linkage group 1 (G1). Five Pru p/du 2 genes were mapped on four different linkage groups, two very similar Pru p/du 2. 01 genes (A and B) were on G3, Pru p/du 2. 02 on G7,Pru p/du 2. 03 on G8 and Pru p/du 2. 04 on G1. There were differences in the intron and exon structure in these Pru p/du 2 genes and in their amino acid composition. Three Pru p/du 3 genes (3. 01-3. 03) containing an intron and a mini exon of 10 nt were mapped in a cluster on G6. Two Pru p/du 4 genes (Pru p/du 4. 01 and 4. 02) were located on G1 and G7, respectively. The Pru p/du 1 cluster on G1 aligned to the Mal d 1 clusters on LG16; Pru p/du 2. 01A and B on G3 to Mal d 2. 01A and B on LG9; the Pru p/du 3 cluster on G6 to Mal d 3. 01 on LG12; Pru p/du 4. 01 on G1 to Mal d 4. 03 on LG2; and Pru p/du 4. 02 on G7 to Mal d 4. 02 on LG2. Conclusion: A total of 18 putative peach/almond allergen genes have been mapped on five linkage groups. Their positions confirm the high macro-synteny between peach/almond and apple. The insight gained will help to identify key genes causing differences in allergenicity among different cultivars of peach and other Prunus species.
Grants:	European Commission 016279
Rights:	Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.
Language:	Anglès
Document:	Article ; recerca ; Versió publicada
Published in:	BMC genomics, Vol. 9 (November 2008) , art. 543, ISSN 1471-2164

DOI: 10.1186/1471-2164-9-543
PMID: 19014629

15 p, 2.4 MB

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Research literature > UAB research groups literature > Research Centres and Groups (research output) > Experimental sciences > CRAG (Centre for Research in Agricultural Genomics)
Articles > Research articles
Articles > Published articles