Web of Science: 3 citations, Scopus: 3 citations, Google Scholar: citations,
Specific activity of superoxide dismutase in stallion seminal plasma is related to sperm cryotolerance
Papas, Marion (Equine Reproduction Service. Department of Animal Medicine and Surgery. Faculty of Veterinary Sciences. Autonomous University of Barcelona)
Catalán, Jaime (Universitat Autònoma de Barcelona. Departament de Cirurgia i Medicina Animals)
Fernandez-Fuertes, Beatriz (Universitat de Girona. Departament de Biologia)
Arroyo, Laura. (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Bassols Teixidó, Anna Maria (Universitat Autònoma de Barcelona. Departament de Bioquímica i de Biologia Molecular)
Miró, Jordi (Universitat Autònoma de Barcelona. Departament de Cirurgia i Medicina Animals)
Yeste, Marc (Universitat de Girona. Departament de Biologia)

Date: 2019
Abstract: While the removal of seminal plasma is a routine practice prior to equine sperm cryopreservation, this fluid contains the main source of antioxidant enzymes able to scavenge these reactive oxygen species. Therefore, stallion seminal plasma components may have an impact on ejaculate freezability. Against this background, this study was designed to investigate whether the activities of the main stallion seminal plasma antioxidant enzymes are related to sperm cryotolerance. With this purpose, 16 ejaculates were collected from 14 healthy stallions, and each ejaculate was split into two aliquots. The first one was used to evaluate the activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), and glutathione reductase (GSR) in seminal plasma. The second aliquot was extended and then processed for cryopreservation. Sperm motility and viability were evaluated before and after cryopreservation, and ejaculates were classified as of good (GFE) or poor freezability (PFE) based on total motile and viable spermatozoa at post-thaw. We observed that, while the specific activities of CAT, GPX, and GSR were similar between GFE and PFE, that of SOD was significantly (p < 0. 05) higher in GFE than in PFE. We can thus conclude that, in stallions, the specific activity of SOD in the seminal plasma of a given ejaculate might be related to its freezability.
Note: Número d'acord de subvenció Regional Government of Catalonia, Spain/2017-SGR-1229
Note: Número d'acord de subvenció Ministry of Science, Innovation and Universities, Spain/[u'AGL2017-88329-R', u'RYC-2014-15581']
Note: Número d'acord de subvenció EU/H2020-MSCA-IF-79212
Rights: Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original. Creative Commons
Language: Anglès
Document: article ; recerca ; publishedVersion
Subject: Seminal plasma ; Sperm ; Equine ; Cryopreservation ; Antioxidant enzymes
Published in: Antioxidants, Vol. 8 Núm. 11 (november 2019) , p. 539, ISSN 2076-3921

DOI: 10.3390/antiox8110539
PMID: 31717586

13 p, 863.3 KB

The record appears in these collections:
Articles > Research articles
Articles > Published articles

 Record created 2020-01-14, last modified 2020-08-02

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